Document Detail

Laser capture microdissection and cDNA microarrays used to generate gene expression profiles of the rapidly expanding fibre initial cells on the surface of cotton ovules.
MedLine Citation:
PMID:  17636323     Owner:  NLM     Status:  MEDLINE    
Cotton (Gossypium hirsutum L.) fibre initial cells undergo a rapid cellular re-programming around anthesis to form the long cellulose fibres prized for textile manufacture. On the day of anthesis the cotton fibre initial cells balloon out from the ovule surface and so are clearly distinguished from adjacent epidermal pavement cells. To enhance our understanding of the molecular processes that determine which cells become fibres and why adjacent epidermal cells remain in a different developmental state we studied the expression profiles of the two respective cell types. Using laser-capture microdissection, coupled with an in vitro RNA amplification system, we used cDNA microarray slides to profile the gene expression in expanding fibre initials compared to the non-expanding epidermal cells at an early stage just after the fibre initials are discernable. Except for a few regulatory genes, the genes that are up-regulated in the cotton fibre initials relative to epidermal cells predominantly encode proteins involved in generating the components for the extra cell membrane and primary cell wall needed for the rapid cell expansion of the initials. This includes synthesis of enzymes and cell wall proteins, carbohydrates, and lipids. An analysis of single channel fluorescence levels confirmed that these classes of genes were also the most highly expressed genes in fibre initials. Genes involved in DNA metabolism were also well represented in the expanding fibre cell, consistent with the limited endoreduplication we previously reported to occur in fibre initial cells.
Yingru Wu; Danny J Llewellyn; Rosemary White; Katya Ruggiero; Yves Al-Ghazi; Elizabeth S Dennis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-18
Journal Detail:
Title:  Planta     Volume:  226     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-11     Completed Date:  2008-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1475-90     Citation Subset:  IM    
CSIRO Plant Industry, P.O. Box 1600, Canberra, ACT 2601, Australia.
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MeSH Terms
Flowers / cytology,  genetics*,  growth & development
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gossypium / cytology,  genetics*,  growth & development
Microdissection / instrumentation,  methods
Oligonucleotide Array Sequence Analysis / methods*
Plant Epidermis / cytology,  genetics,  growth & development

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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