Document Detail

Laser-assisted removal of necrotic blastomeres from cryopreserved embryos that were partially damaged.
MedLine Citation:
PMID:  12057728     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine whether the developmental potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres before embryo transfer. DESIGN: Prospective pilot study and observational clinical series. SETTING: Private hospital. PATIENT(S): Two hundred thirty-five infertile couples undergoing frozen embryo transfer. INTERVENTION(S): Removal of necrotic blastomeres from frozen-thawed human embryos. MAIN OUTCOME MEASURE(S): Pregnancy and implantation rates. RESULT(S): Removal of necrotic blastomeres from partially damaged frozen-thawed embryos before transfer increased rates of pregnancy (45.7% vs. 17.1%), ongoing pregnancy (40.0% vs. 11.4%) and ongoing implantation (16.2% vs. 4.3%) compared with the control group, in which necrotic blastomeres were not removed. A similarly high implantation rate (16.7%) was seen a subsequent clinical series in which necrotic blastomeres were removed from all partially damaged embryos. CONCLUSION(S): The viability of partially damaged frozen-thawed embryos can be improved by removal of necrotic blastomeres before embryo transfer.
Laura Rienzi; Zsolt Peter Nagy; Filippo Ubaldi; Marcello Iacobelli; Reno Anniballo; Jan Tesarik; Ermanno Greco
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Fertility and sterility     Volume:  77     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-11     Completed Date:  2002-07-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1196-201     Citation Subset:  IM    
Centre for Reproductive Medicine, European Hospital, Rome, Italy.
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MeSH Terms
Blastomeres / pathology*,  radiation effects*
Embryo Implantation
Embryo, Mammalian / pathology*,  physiology
Pilot Projects
Pregnancy Rate
Prospective Studies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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