Document Detail


Laser-induced acoustic desorption coupled with a linear quadrupole ion trap mass spectrometer.
MedLine Citation:
PMID:  20000769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, laser-induced acoustic desorption (LIAD) coupled with a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer has been demonstrated to provide a valuable technique for the analysis of a wide variety of nonvolatile, thermally labile compounds, including analytes that could not previously be analyzed by mass spectrometry. Although FT-ICR instruments are very powerful, they are also large and expensive and, hence, mainly used as research instruments. In contrast, linear quadrupole ion trap (LQIT) mass spectrometers are common due to several qualities that make these instruments attractive for both academic and industrial settings, such as high sensitivity, large dynamic range, and experimental versatility. Further, the relatively small size of the instruments, comparatively low cost, and the lack of a magnetic field provide some distinct advantages over FT-ICR instruments. Hence, we have coupled the LIAD technique with a commercial LQIT, the Thermo Fischer Scientific LTQ mass spectrometer. The LQIT was modified for a LIAD probe by outfitting the removable back plate of the instrument with a 6 in. ConFlat flange (CFF) port, gate valve, and sample lock. Reagent ions were created using the LQIT's atmospheric pressure ionization source and trapped in the mass analyzer for up to 10 s to allow chemical ionization reactions with the neutral molecules desorbed via LIAD. These initial experiments focused on demonstrating the feasibility of performing LIAD in the LQIT. Hence, the results are compared to those obtained using an FT-ICR mass spectrometer. Despite the lower efficiency in the transfer of desorbed neutral molecules into the ion trap, and the smaller maximum number of available laser pulses, the intrinsically higher sensitivity of the LQIT resulted in a higher sensitivity relative to the FT-ICR.
Authors:
Steven C Habicht; Lucas M Amundson; Penggao Duan; Nelson R Vinueza; Hilkka I Kenttämaa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  82     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-14     Completed Date:  2010-03-22     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  608-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Angiotensin II / analogs & derivatives,  analysis,  chemistry
Enkephalin, Leucine / analysis,  chemistry
Fourier Analysis
Ions / chemistry*
Lasers*
Mass Spectrometry / instrumentation*,  methods
Peptides / analysis*,  chemistry
Grant Support
ID/Acronym/Agency:
R01 GM052418/GM/NIGMS NIH HHS; R01 GM052418-10/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Ions; 0/Peptides; 11128-99-7/Angiotensin II; 23025-68-5/angiotensin II, des-Asp(1)-des-Arg(2)-Ile(5)-; 58822-25-6/Enkephalin, Leucine
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