Document Detail


Laser Doppler imaging of reactive hyperemia exposes blood flow deficits in a rat model of experimental limb ischemia.
MedLine Citation:
PMID:  19433986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primary pathophysiology of peripheral artery occlusive disease is associated with impaired perfusion to the lower extremities. The lack of effective pharmacologic agents to treat this disease emphasizes the need for well-characterized animal models that can be used to evaluate the efficacy of emerging therapies. A major limitation with the current animal models of peripheral artery occlusive disease is that the variety of surgical methods employed to reduce peripheral blood flow produce differences in the severity and time course of the resting and reserve blood flow deficits. Furthermore, the methods used to evaluate the restoration of peripheral flow are often not suitable for serial measurements. This study used laser Doppler imaging to serially evaluate resting blood flow and the development of a functional collateral circulation after the induction of hind limb ischemia in the rat. Reserve blood flow was assessed by measuring hyperemic blood flow in the hind paw after temporary arterial occlusion. The magnitude of the hyperemic response was found to be dependent upon both the duration of arterial occlusion and the measurement time after release of the occluder. After ligation of the common iliac artery, but at a time when resting blood flow was reestablished, hyperemic tests unmasked a sustained deficit in reserve blood flow capacity that persisted for at least 14 days. Therefore, the use of a noninvasive vascular occluder and laser Doppler imaging represents a sensitive and consistent technique to measure peripheral blood flow status to assess the development of functional collateral vessels. These findings will enhance the ability to effectively study pharmacologic therapies aimed at promoting the growth and development of collateral vessels.
Authors:
Halea A Corcoran; Brad E Smith; Parker Mathers; Dan Pisacreta; James C Hershey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  53     ISSN:  1533-4023     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  446-51     Citation Subset:  IM    
Affiliation:
Department of Molecular Endocrinology, Merck Research Laboratories, West Point, PA 19454, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Collateral Circulation
Disease Models, Animal
Hindlimb / blood supply*
Hyperemia / physiopathology*
Ischemia / physiopathology*
Laser-Doppler Flowmetry
Male
Microcirculation
Muscle, Skeletal / blood supply*
Rats
Rats, Sprague-Dawley
Regional Blood Flow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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