Document Detail

Laser Doppler imaging of reactive hyperemia exposes blood flow deficits in a rat model of experimental limb ischemia.
MedLine Citation:
PMID:  19433986     Owner:  NLM     Status:  MEDLINE    
The primary pathophysiology of peripheral artery occlusive disease is associated with impaired perfusion to the lower extremities. The lack of effective pharmacologic agents to treat this disease emphasizes the need for well-characterized animal models that can be used to evaluate the efficacy of emerging therapies. A major limitation with the current animal models of peripheral artery occlusive disease is that the variety of surgical methods employed to reduce peripheral blood flow produce differences in the severity and time course of the resting and reserve blood flow deficits. Furthermore, the methods used to evaluate the restoration of peripheral flow are often not suitable for serial measurements. This study used laser Doppler imaging to serially evaluate resting blood flow and the development of a functional collateral circulation after the induction of hind limb ischemia in the rat. Reserve blood flow was assessed by measuring hyperemic blood flow in the hind paw after temporary arterial occlusion. The magnitude of the hyperemic response was found to be dependent upon both the duration of arterial occlusion and the measurement time after release of the occluder. After ligation of the common iliac artery, but at a time when resting blood flow was reestablished, hyperemic tests unmasked a sustained deficit in reserve blood flow capacity that persisted for at least 14 days. Therefore, the use of a noninvasive vascular occluder and laser Doppler imaging represents a sensitive and consistent technique to measure peripheral blood flow status to assess the development of functional collateral vessels. These findings will enhance the ability to effectively study pharmacologic therapies aimed at promoting the growth and development of collateral vessels.
Halea A Corcoran; Brad E Smith; Parker Mathers; Dan Pisacreta; James C Hershey
Related Documents :
2787546 - Hemodilution increases cerebral blood flow in acute ischemic stroke.
11950646 - Time-threshold curve determined by single photon emission ct in patients with acute mid...
1691396 - Effect of long-term oral nisoldipine on infarct size and collateral development in pigs...
16645136 - Cortical perfusion measurement by indocyanine-green videoangiography in patients underg...
3168266 - Screening of velopharyngeal closure based on nasal airflow rate measurements.
19988216 - Discussion on the pathology and treatment of haemopathies, including leukaemias, of man...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  53     ISSN:  1533-4023     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  446-51     Citation Subset:  IM    
Department of Molecular Endocrinology, Merck Research Laboratories, West Point, PA 19454, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Collateral Circulation
Disease Models, Animal
Hindlimb / blood supply*
Hyperemia / physiopathology*
Ischemia / physiopathology*
Laser-Doppler Flowmetry
Muscle, Skeletal / blood supply*
Rats, Sprague-Dawley
Regional Blood Flow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Myocardial homing and coronary endothelial function after autologous blood CD34+ progenitor cells in...
Next Document:  Crying and feeding problems in infancy and cognitive outcome in preschool children born at risk: a p...