Document Detail


Laser Doppler flowmetry of hepatic microcirculation during Pringle's maneuver: determination of spatial and temporal liver tissue perfusion heterogeneity.
MedLine Citation:
PMID:  20215755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Laser Doppler flowmetry (LDF) is frequently used for non-invasive microvascular perfusion measurements. The aim of the present study was to analyze liver blood flow heterogeneity in detail using LDF devices under normal and low-flow conditions.
MATERIALS AND METHODS: In 5 anesthetized and laparotomized Suabian-Hall strain pigs, systemic hemodynamics and hepatic arterial/portal venous blood flow were constantly recorded. Hepatic microcirculation was assessed by 2 different LDF devices, analyzing microvascular flow and velocity before, during and after inducing a Pringle's maneuver for hepatic inflow occlusion. Offline data analysis comprised differentiation between the two LDF devices used as well as calculation of temporal and spatial heterogeneity of liver perfusion.
RESULTS: Pringle's maneuver induced complete inflow occlusion, confirmed by hepatic arterial/portal venous blood flow measurement. Laser Doppler signals showed a significant decrease during Pringle's maneuver. Spatial heterogeneity of flow and velocity increased more than temporal heterogeneity during Pringle's maneuver.
CONCLUSION: Both LDF devices proved suitable for assessing hepatic microvascular perfusion during normal perfusion and low-flow conditions. Reduced microvascular perfusion induces a significant increase in temporal and spatial perfusion heterogeneity. In particular, the pronounced spatial heterogeneity requires measurements at different places when assessing hepatic microcirculation by LDF during impaired perfusion conditions.
Authors:
S Richter; J Sperling; O Kollmar; M D Menger; M K Schilling;
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Publication Detail:
Type:  Journal Article     Date:  2010-03-09
Journal Detail:
Title:  European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes     Volume:  44     ISSN:  1421-9921     ISO Abbreviation:  Eur Surg Res     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0174752     Medline TA:  Eur Surg Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  152-8     Citation Subset:  IM    
Copyright Information:
(c) 2010 S. Karger AG, Basel.
Affiliation:
Department of General, Visceral, Vascular and Pediatric Surgery, University of Saarland, Homburg/Saar, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Hemodynamics
Hepatic Artery / physiology,  ultrasonography
Laser-Doppler Flowmetry
Liver / blood supply*,  injuries,  ultrasonography*
Liver Circulation / physiology*
Microcirculation / physiology*
Models, Animal
Portal Vein / physiology,  ultrasonography
Reperfusion Injury / physiopathology,  ultrasonography
Sus scrofa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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