Document Detail

Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity.
MedLine Citation:
PMID:  833209     Owner:  NLM     Status:  MEDLINE    
The application of roller-bottle cell culture techniques and a relatively simple purification scheme has led to the isolation of milligram quantities of a polypeptide cell multiplication stimulating activity (MSA) from Buffalo rat liver cell conditioned medium. We have characterized the apparently homogeneous MSA with respect to its biological activity, its N-terminal amino acid residue, and its amino acid composition, and have tested the MSA for growth-promoting activity in a number of cell types.
N C Dulak; Y W Shing
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  90     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-03-31     Completed Date:  1977-03-31     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  127-37     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Cell Division / drug effects
Cell Line
Culture Media*
Electrophoresis, Polyacrylamide Gel
Growth Substances / analysis,  isolation & purification*,  pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Growth Substances

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