| Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. | |
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MedLine Citation:
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PMID: 833209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The application of roller-bottle cell culture techniques and a relatively simple purification scheme has led to the isolation of milligram quantities of a polypeptide cell multiplication stimulating activity (MSA) from Buffalo rat liver cell conditioned medium. We have characterized the apparently homogeneous MSA with respect to its biological activity, its N-terminal amino acid residue, and its amino acid composition, and have tested the MSA for growth-promoting activity in a number of cell types. |
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Authors:
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N C Dulak; Y W Shing |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular physiology Volume: 90 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1977 Jan |
Date Detail:
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Created Date: 1977-03-31 Completed Date: 1977-03-31 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 127-37 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Cell Division / drug effects Cell Line Culture Media* Electrophoresis, Polyacrylamide Gel Growth Substances / analysis, isolation & purification*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 0/Growth Substances |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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