Document Detail

Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up.
MedLine Citation:
PMID:  6584068     Owner:  NLM     Status:  MEDLINE    
Mammalian cell products have great medical and clinical importance, but to date, production methods employed to manufacture these products on a large scale are not as cost efficient as they could be. The implementation of process control would greatly improve the productivity of these products. Recently developed methods to produce cells on a large scale, such as microcarriers, artificial capillaries, tubular spiral film, and microencapsulation must be optimized, and the problem of oxygen transfer limitation must be solved. The accumulation of potentially toxic waste products can inhibit growth and reduce productivity. This effect can be reduced by either adjusting the environmental parameters of a fed-batch culture, so that the cell's metabolism is shifted away from producing these compounds, or by continually perfusing medium through the culture. If these technical barriers can be overcome, the cost of producing products derived from mammalian cells can be greatly reduced.
M W Glacken; R J Fleischaker; A J Sinskey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  413     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1983  
Date Detail:
Created Date:  1984-03-26     Completed Date:  1984-03-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  355-72     Citation Subset:  IM    
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MeSH Terms
Biomedical Engineering / instrumentation
Cell Adhesion
Cell Physiological Phenomena*
Cells, Cultured
Culture Techniques / methods
Grant Support
5-T32-CA09258-05/CA/NCI NIH HHS

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