Document Detail


Large-scale preparation of the oligosaccharide phosphate fraction of Pichia holstii NRRL Y-2448 phosphomannan for use in the manufacture of PI-88.
MedLine Citation:
PMID:  11434376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 C 3)-alpha-D-Man-(1 --> 2)-D-Man, for use in the manufacture of the promising new anti-cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction.
Authors:
V Ferro; K Fewings; M C Palermo; C Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  332     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-03     Completed Date:  2001-12-13     Revised Date:  2005-06-17    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
Affiliation:
Department of Research and Development, Progen Industries Ltd, Richlands BC, Qld, Australia. vito.ferro@progen.com.au
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / chemical synthesis*
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Hydrolysis
Industrial Microbiology / methods
Mannans / chemistry,  isolation & purification*
Molecular Sequence Data
Molecular Weight
Nuclear Magnetic Resonance, Biomolecular
Oligosaccharides / chemical synthesis*,  chemistry,  isolation & purification*
Pichia / chemistry*
Chemical
Reg. No./Substance:
0/6-phosphomannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-2-mannose; 0/Antineoplastic Agents; 0/Mannans; 0/Oligosaccharides; 0/phosphomannopentaose sulfate; 9044-08-0/phosphomannan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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