Document Detail


Large-scale identification of gibberellin-related transcription factors defines Group VII ERFs as functional DELLA partners.
MedLine Citation:
PMID:  25118255     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
DELLA proteins are the master negative regulators in gibberellin (GA) signaling acting in the nucleus as transcriptional regulators. The current view of DELLA action indicates that their activity relies on the physical interaction with transcription factors (TFs). Therefore, the identification of TFs through which DELLAs regulate GA responses is key to understand these responses from a mechanistic point of view. Here, we have determined the TF-interactome of the Arabidopsis (Arabidopsis thaliana) DELLA protein GIBBERELLIN INSENSITIVE (GAI), and screened a collection of conditional TF overexpressors in search for those that alter GA sensitivity. As a result, we have found RELATED TO APETALA2.3 (RAP2.3), an ethylene-induced TF belonging to the Group VII ETHYLENE RESPONSE FACTOR (ERF) of the AP2/ERBP superfamily, as a DELLA interactor with physiological relevance in the context of apical hook development. The combination of transactivation assays and chromatin immunoprecipitation indicates that the interaction with GAI impairs the activity of RAP2.3 on the target promoters. This mechanism represents a novel node in the cross-regulation between the GA and ethylene signaling pathways controlling differential growth during apical hook development.
Authors:
Nora Marin-de la Rosa; Berta Sotillo; Pal Miskolczi; Daniel J Gibbs; Jorge Vicente; Pilar Carbonero; Luis Onate-Sanchez; Michael J Holdsworth; Rishikesh Bhalerao; David Alabadi; Miguel A Blazquez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-12
Journal Detail:
Title:  Plant physiology     Volume:  -     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society of Plant Biologists.
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