| Large-scale extraction of recombinant proteins from bacteria. | |
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MedLine Citation:
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PMID: 20810631 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Bacteria are particularly convenient for producing recombinant proteins for purification purposes. Suitable extraction methods for bacterial cells include sonication, glass bead milling, grinding with alumina or sand, high-pressure shearing using the French pressure cell (French Press), and lysozyme treatment. These procedures are applicable for preparing extracts from a variety of gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae, and gram-positive bacteria such as Bacillus subtilis. Disruption of bacterial cells by enzymatic means is commonly used because a relatively uniform treatment is obtained when cells are in suspension. This protocol describes the enzymatic disruption of E. coli. |
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Authors:
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Richard J Simpson |
Publication Detail:
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Type: Journal Article Date: 2010-09-01 |
Journal Detail:
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Title: Cold Spring Harbor protocols Volume: 2010 ISSN: 1559-6095 ISO Abbreviation: Cold Spring Harb Protoc Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101524530 Medline TA: Cold Spring Harb Protoc Country: United States |
Other Details:
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Languages: eng Pagination: pdb.prot5484 Citation Subset: IM |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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