Document Detail

Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection.
MedLine Citation:
PMID:  23132278     Owner:  NLM     Status:  Publisher    
A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties.
Marit H Stafsnes; Marius Dybwad; Anders Brunsvik; Per Bruheim
Related Documents :
24078078 - Leading the herd astray: an experimental study of self-fulfilling prophecies in an arti...
24515908 - Time-lapse imaging of preimplantation embryos.
23174778 - Production of myostatin-targeted goat by nuclear transfer from cultured adult somatic c...
24305338 - 155 effect of co-culture with cumulus-derived somatic cells during in vitro maturation ...
4020138 - Immune oxidative injury induced in mice exposed to normobaric o2: effects of thiol comp...
2536648 - Age as a function of atpase activity in cultured corneal endothelial cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-7
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  -     ISSN:  1572-9699     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biotechnology, Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Selection and characterization of a newly isolated thermotolerant Pichia kudriavzevii strain for eth...
Next Document:  Spongiimonas flava gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a...