| Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection. | |
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MedLine Citation:
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PMID: 23132278 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties. |
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Authors:
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Marit H Stafsnes; Marius Dybwad; Anders Brunsvik; Per Bruheim |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-7 |
Journal Detail:
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Title: Antonie van Leeuwenhoek Volume: - ISSN: 1572-9699 ISO Abbreviation: Antonie Van Leeuwenhoek Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372625 Medline TA: Antonie Van Leeuwenhoek Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biotechnology, Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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