Document Detail


Large gradient high magnetic field affects FLG29.1 cells differentiation to form osteoclast-like cells.
MedLine Citation:
PMID:  22642494     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Purpose: We aimed to investigate the effects of different apparent gravities (μg, 1g and 2g) produced by large gradient high magnetic field (LGHMF) on human preosteoclast FLG29.1 cells. Materials and methods: FLG29.1 cells were cultured in Roswell Park Memorial Institute (RPMI) -1640 medium. Cells were exposed to LGHMF for 72 h. On culture day 1, 2, 3, cell proliferation was detected by 3-(4,5)-dimethylthiahi-azo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. On day 3, cell apoptosis and necrosis were assayed by Hoechst and propidium iodide (PI) staining. After cells were exposed to LGHMF for 72 h with the induction of 12-o-tetradecanoylphorbol 13-acetate (TPA), Tartrate-Resistant Acid Phosphatase (TRAP) positive cells and nitric oxide (NO) release were detected by TRAP staining and Griess method, respectively. Intracellular TRAP activity was measured using nitrophenylphosphate (pNPP) as the substrate. Results: MTT detection revealed that compared to control, FLG 29.1 cell proliferation in μg and 2g groups were promoted. However, there is no obvious difference between 1g and control groups. Hoechst-PI staining showed that LGHMF promoted cell apoptosis and necrosis, especially in 2g group. Exposure to LGHMF inhibited the NO concentration of supernatant. Both the TRAP activity and the number of TRAP positive cells were higher in cells of μg group than those in 2g group. In 1g group, they were decreased significantly compared to control. Conclusions: These findings indicate that LGHMF could directly affect human preosteoclast FLG29.1 cells survival and differentiation. High magnetic flux inhibited osteoclasts formation and differentiation while reduced apparent gravity enhanced osteoclastogenesis.
Authors:
Shengmeng Di; Zongcheng Tian; Airong Qian; Jingbao Li; Jiawei Wu; Zhe Wang; Dayu Zhang; Dachuan Yin; Maria Luisa Brandi; Peng Shang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-29
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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