| Large differences in erythrocyte stability between species reflect different antioxidative defense mechanisms. | |
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MedLine Citation:
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PMID: 12180129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a screening assay for erythrocyte stability, which is rapid, easy, inexpensive, robust, and suitable for handling a large number of samples in parallel. Erythrocytes are incubated overnight in 96-well microtiter plates in absence or presence of various oxidants, intact cells are pelleted by centrifugation, and lysis is determined by release of intracellular constituents into the supernatant as either activity of lactate dehydrogenase (LDH) or absorbance of hemoglobin at 406 nm. There is good correlation between the methods. A number of advantages by the present method are that only small amounts of blood is needed, washing is optional, erythrocytes may be stored for at least one day before assay, and large numbers of samples can be handled in parallel. Using this set-up, we have compared erythrocyte stability from several different animal species. We find that erythrocyte susceptibility towards lysis induced by H2O2 and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) is highly species dependent. The different susceptibility between species is due to cellular components, since swapping of plasma between species has little or no effect. As a novel observation, we find that erythrocytes from chicken are the most sensitive of the species tested towards lysis by H2O2 and are almost four orders of magnitude more sensitive than erythrocytes from man. This is due to a much lower content of catalase in erythrocytes from chicken. A more narrow range is observed for susceptibility towards AAPH and the ranking between the species is different. Thus, chicken erythrocytes are more resistant towards AAPH than some mammals by up to two orders of magnitude. This differential stability towards different oxidative stressors is likely due to evolution/selection of different defense mechanisms. |
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Authors:
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Jan Stagsted; Jette F Young |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 36 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-08-15 Completed Date: 2003-01-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 779-89 Citation Subset: IM |
Affiliation:
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Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark. Jan.Stagsted@agrsci.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amidines
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pharmacology Animals Antioxidants / metabolism* Birds Catalase / metabolism Cats Cattle Chickens Erythrocyte Membrane / drug effects, enzymology Erythrocytes / physiology* Hemoglobins / metabolism Humans Hydrogen Peroxide / pharmacology L-Lactate Dehydrogenase / metabolism Oxidative Stress / physiology* Rabbits Species Specificity Swine |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Antioxidants; 0/Hemoglobins; 13217-66-8/2,2'-azobis(2-amidinopropane); 7722-84-1/Hydrogen Peroxide; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.11.1.6/Catalase |
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