| Lansoprazole Inhibits Nitric Oxide and Prostaglandin E(2) Production in Murine Macrophage RAW 264.7 Cells. | |
| | |
MedLine Citation:
|
PMID: 22134422 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Aberrantly activated macrophages, which overproduce inflammatory mediators, are involved in the pathogenesis of many inflammatory diseases. We analyzed the anti-inflammatory activity of lansoprazole (LPZ), a typical proton pump (P-ATPase) inhibitor, on RAW264.7 murine macrophages. Treatment of lipopolysaccharide (LPS)-stimulated RAW264.7 cells with LPZ inhibited the production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)). Since P-ATPase expression was not observed in RAW264.7 cells, the anti-inflammatory effect of LPZ was independent of ATPase. In contrast, diphenylene iodonium (DPI), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, decreased NO but not PGE(2) levels. LPZ suppressed the LPS-stimulated production by RAW264.7 cells of reactive oxygen species (ROS), which plays an important role in inflammatory responses. ROS elevation in these cells was associated with NO but not PGE(2) production, suggesting that LPZ inhibits NO production by suppressing NADPH oxidase activity. These findings suggest that LPZ may be useful in the treatment of many inflammatory diseases associated with activated macrophages. |
| | |
Authors:
|
Shuji Nakagawa; Yuji Arai; Tsunao Kishida; Nobuyuki Hiraoka; Shinji Tsuchida; Hiroaki Inoue; Ryo Sakai; Osam Mazda; Toshikazu Kubo |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-2 |
Journal Detail:
|
Title: Inflammation Volume: - ISSN: 1573-2576 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600105 Medline TA: Inflammation Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Emergency physicians' opinions on the use of intravenous fluids to treat patients intoxicated with e...
Next Document: Highly ordered mesoporous Cr(2)O(3) materials with enhanced performance for gas sensors and lithium ...