Document Detail

Lanosterol 14alpha-demethylase expression in the mouse ovary and its participation in cumulus-enclosed oocyte spontaneous meiotic maturation in vitro.
MedLine Citation:
PMID:  16650467     Owner:  NLM     Status:  MEDLINE    
The expression of lanosterol 14alpha-demethylase (LDM) in the mouse ovary after gonadotrophin administration was examined and the action of follicle fluid meiosis activating sterol (FF-MAS), derived from lanosterol by the action of LDM, on oocyte spontaneous maturation was also evaluated in cumulus cell enclosed oocytes (CEOs). Expression of LDM was primarily in oocytes in primordial and secondary follicles prior to administration of gonadotrophins, but obvious LDM expression was apparent in ovarian somatic cells 48 h after administration of equine chorionic gonadotrophin (eCG), especially in luteal and cumulus cells 54 h after eCG or 48 h after eCG plus 6 h after human chorionic gonadotrophin (hCG). The LDM expression in oocytes was only slightly elevated in larger growing follicles after eCG treatment. On the contrary, 48 h after hCG treatment, the elevated expression of LDM was only detected in interstitial cells. Therefore, eCG may be the primary gonadotrophin for LDM expression, and furthermore for production of FF-MAS in mouse cumulus cells (which are indispensable for oocyte maturation in vivo). Conversely, inhibitors of LDM, either 40 microM azalanstat or 50 microM RS-21745, significantly inhibited oocyte germinal vesicle breakdown (GVB) after 4h of in vitro culture; GVB rates decreased to 14 or 20%, compared to 90% in spontaneous maturation, respectively. There was no significant increase in GVB in CEOs following specific inhibitor of sterol Delta14-reductase and Delta7-reductase, AY9944-A-7 (5-100 microM), until marked oocytes degeneration appeared (50 microM). The phenomena may be ascribed to slow, passive accumulation of FF-MAS by AY9944-A-7, which cannot be associated with fast spontaneous progression. Furthermore, in spontaneous-matured CEOs, LDM was expressed preferentially in cumulus cells instead of oocytes. Therefore, FF-MAS may have a positive role in the spontaneous maturation of CEOs. In conclusion, there was an eCG-dependent dual LDM expression pattern on both oocytes and somatic cells in growing follicles in vivo, which may increase LDM expression and FF-MAS production in cumulus cells for oocyte maturation. For the first time, the inhibitory effect of LDM inhibitors on spontaneous maturation, together with the strong LDM expression in spontaneous matured CEOs, indicated that FF-MAS produced by cumulus cells might participate in spontaneous maturation of mouse CEOs.
Chao Wang; Huirong Xie; Xiaoming Song; Gang Ning; Jun Yan; Xiufen Chen; Baoshan Xu; Hong Ouyang; Guoliang Xia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-02
Journal Detail:
Title:  Theriogenology     Volume:  66     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2007-07-12     Revised Date:  2010-04-06    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1156-64     Citation Subset:  IM    
Department of Animal Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
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MeSH Terms
Aniline Compounds
Chorionic Gonadotropin / pharmacology
Cytochrome P-450 Enzyme System / antagonists & inhibitors,  metabolism*
Enzyme Inhibitors / pharmacology
Gonadotropins / pharmacology*
Gonadotropins, Equine / pharmacology
Meiosis / drug effects*,  physiology
Oocytes / drug effects,  physiology*
Ovary / enzymology*
Oxidoreductases / antagonists & inhibitors,  metabolism*
Sexual Maturation
Reg. No./Substance:
0/Aniline Compounds; 0/Chorionic Gonadotropin; 0/Enzyme Inhibitors; 0/Gonadotropins; 0/Gonadotropins, Equine; 0/Sterols; 0/Sulfides; 143393-27-5/azalanstat; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 14-demethylase

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