Document Detail


Langerhans cells are not required for epidermal Vgamma3 T cell homeostasis and function.
MedLine Citation:
PMID:  21486908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study tested the hypothesis that Vγ3 TCR-bearing T cells are influenced by LCs. Vγ3 T cells and LCs are located in the epidermis of mice. Vγ3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although Vγ3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of Vγ3 T cells. We show that Vγ3 T cell numbers are unaltered by LC deficiency, and Vγ3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from Vγ3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skin-wounding experiments show no major difference in response of Vγ3 T cells to wounding in the absence or presence of LCs. These observations indicate that Vγ3 T cells develop and function independently of LCs.
Authors:
Sylvie Taveirne; Veerle De Colvenaer; Tina Van Den Broeck; Els Van Ammel; Clare L Bennett; Tom Taghon; Bart Vandekerckhove; Jean Plum; Björn E Clausen; Daniel H Kaplan; Georges Leclercq
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-12
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  90     ISSN:  1938-3673     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-09-09     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
Affiliation:
Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / immunology
Cell Separation
Epidermis / cytology,  immunology
Flow Cytometry
Homeostasis / immunology*
Immunohistochemistry
Langerhans Cells / cytology,  immunology*
Mice
Mice, Transgenic
Receptors, Antigen, T-Cell, gamma-delta / immunology*
Skin / cytology,  immunology*
T-Lymphocytes / cytology,  immunology*
Grant Support
ID/Acronym/Agency:
AR044077/AR/NIAMS NIH HHS; R01 AR056632/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Antigen, T-Cell, gamma-delta
Comments/Corrections

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