Document Detail

Lamprey (Lethenteron japonicum) IL-17 upregulated by LPS-stimulation in the skin cells.
MedLine Citation:
PMID:  17924104     Owner:  NLM     Status:  MEDLINE    
We report here the first evidence for interleukin-17, a pro-inflammatory cytokine, in cyclostomes. To detect the novel molecules involved in the immune response in the skin of the lamprey Lethenteron japonicum, subtractive hybridization was performed with 6-h-cultured skin cells with or without lipopolysaccharide (LPS). In approximately 100 partially sequenced clones analyzed, we identified an interesting sequence similar to that of the IL-17 genes in teleosts and mammals. Subsequent rapid amplification of cDNA ends was used to obtain the cDNA of lamprey IL-17 (LampIL-17) that contains a 519-bp open reading frame encoding a mature protein of 154 amino acids and a 19-residue NH2-terminal signal peptide. The phylogenetic tree indicated that LampIL-17 is clustered into IL-17D, which is a subgroup of the IL-17 family. Southern blot analysis showed that the lamprey harbors a single copy of the LampIL-17 gene in its genome. The LampIL-17 gene was constitutively expressed in most tissues examined as well as in the skin, where the basal layer epithelial cells expressed LampIL-17 mRNA. Real-time-polymerase chain reaction (RT-PCR) demonstrated that the LampIL-17 gene expression in LPS-stimulated skin cells tended to be greater than that in non-stimulated cells. These results suggest that LampIL-17 is responsible for defense against bacterial infections in the lamprey skin.
Shigeyuki Tsutsui; Osamu Nakamura; Tasuku Watanabe
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-09
Journal Detail:
Title:  Immunogenetics     Volume:  59     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  873-82     Citation Subset:  IM    
School of Fisheries Science, Kitasato University, Sanriku, Ofunato, Iwate 022-0101, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Primers / genetics
DNA, Complementary / genetics
Gene Dosage
Interleukin-17 / genetics*
Lampreys / anatomy & histology,  genetics*,  immunology*
Lipopolysaccharides / pharmacology
Molecular Sequence Data
RNA, Messenger / genetics,  metabolism
Sequence Homology, Amino Acid
Skin / cytology,  drug effects,  immunology*
Up-Regulation / drug effects
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/Interleukin-17; 0/Lipopolysaccharides; 0/RNA, Messenger

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