Document Detail


Laminins 2 and 4 are expressed by human decidual cells.
MedLine Citation:
PMID:  8569185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During pregnancy, the resident stromal cells of the endometrium differentiate to become decidual cells and produce a pericellular basement membrane. We used immunofluorescence and Western blotting with a panel of monoclonal Ab specific for various laminin subunits to examine the composition of decidual laminin. The stromal cell basement membrane contained subunits alpha 2 (M), beta 1 (B1), beta 2 (S), and gamma 1 (B2). Low levels of alpha 1 could also be detected. The glandular and vascular basement membranes of decidual tissue contained subunits alpha 1 (A), beta 1, and gamma 1. An extract was produced from decidual extracellular matrix. Western blots of nonreducing gels showed the presence of high molecular weight complexes containing alpha 2, beta 1, beta 2, and gamma 1. These data indicated that laminins 2 and 4 are coexpressed by decidual cells. Laminin 1 was present in the extract as a minor component. In contrast, cultured stromal cells expressed laminin 1 as the major secreted variant. Immunolocalization was carried out using tissue from various stages of the nonpregnant cycle. The alpha 2 chain polypeptide was absent in the proliferative phase of the cycle but present in late secretory phase in perivascular areas where predecidual differentiation occurs. Reverse transcriptase-PCR experiments confirmed the presence of alpha 2 chain mRNA in decidua but showed that this transcript is detectable throughout the nonpregnant cycle. The results showed that laminins 2 and 4 are hormonally regulated products of decidual cells. The composition of the vascular and epithelial basement membranes remained constant throughout the cycle.
Authors:
H J Church; L M Vićovac; J D Williams; N A Hey; J D Aplin
Related Documents :
17360445 - Interaction with the mammary microenvironment redirects spermatogenic cell fate in vivo.
10406075 - The decidua regulates hemostasis in human endometrium.
11171335 - Parathyroid hormone-related protein maintains mammary epithelial fate and triggers nipp...
25488725 - Hemicellulosic ethanol production by immobilized cells of scheffersomyces stipitis: eff...
8413615 - Residues in the tata-binding protein required to mediate a transcriptional response to ...
24346035 - The role of iron and reactive oxygen species in cell death.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  74     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-03-04     Completed Date:  1996-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-32     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cells, Cultured
Decidua / chemistry,  cytology,  metabolism*
Endometrium / chemistry,  cytology,  metabolism
Female
Humans
Immunohistochemistry
Laminin / biosynthesis*,  chemistry,  genetics
Peptides / metabolism
Pregnancy
RNA, Messenger / analysis
Stromal Cells / metabolism
Chemical
Reg. No./Substance:
0/Laminin; 0/Peptides; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Overexpression of surfactant protein SP-A, SP-B, and SP-C mRNA in rat lungs with lipopolysaccharide-...
Next Document:  Experimental reovirus serotype 1/strain Lang infection of the lung: a model for the study of the lun...