Document Detail

Laminins 2 and 4 are expressed by human decidual cells.
MedLine Citation:
PMID:  8569185     Owner:  NLM     Status:  MEDLINE    
During pregnancy, the resident stromal cells of the endometrium differentiate to become decidual cells and produce a pericellular basement membrane. We used immunofluorescence and Western blotting with a panel of monoclonal Ab specific for various laminin subunits to examine the composition of decidual laminin. The stromal cell basement membrane contained subunits alpha 2 (M), beta 1 (B1), beta 2 (S), and gamma 1 (B2). Low levels of alpha 1 could also be detected. The glandular and vascular basement membranes of decidual tissue contained subunits alpha 1 (A), beta 1, and gamma 1. An extract was produced from decidual extracellular matrix. Western blots of nonreducing gels showed the presence of high molecular weight complexes containing alpha 2, beta 1, beta 2, and gamma 1. These data indicated that laminins 2 and 4 are coexpressed by decidual cells. Laminin 1 was present in the extract as a minor component. In contrast, cultured stromal cells expressed laminin 1 as the major secreted variant. Immunolocalization was carried out using tissue from various stages of the nonpregnant cycle. The alpha 2 chain polypeptide was absent in the proliferative phase of the cycle but present in late secretory phase in perivascular areas where predecidual differentiation occurs. Reverse transcriptase-PCR experiments confirmed the presence of alpha 2 chain mRNA in decidua but showed that this transcript is detectable throughout the nonpregnant cycle. The results showed that laminins 2 and 4 are hormonally regulated products of decidual cells. The composition of the vascular and epithelial basement membranes remained constant throughout the cycle.
H J Church; L M Vićovac; J D Williams; N A Hey; J D Aplin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  74     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-03-04     Completed Date:  1996-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-32     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
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MeSH Terms
Blotting, Western
Cells, Cultured
Decidua / chemistry,  cytology,  metabolism*
Endometrium / chemistry,  cytology,  metabolism
Laminin / biosynthesis*,  chemistry,  genetics
Peptides / metabolism
RNA, Messenger / analysis
Stromal Cells / metabolism
Reg. No./Substance:
0/Laminin; 0/Peptides; 0/RNA, Messenger

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