Document Detail


Laminin from rat yolk sac tumor: isolation, partial characterization, and comparison with mouse laminin.
MedLine Citation:
PMID:  6847209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laminin was isolated from a rat yolk sac tumor by salt extraction, gel filtration, and affinity chromatography on heparin-Sepharose. The purified laminin gave two polypeptide chains with approximate Mr of 200,000 and 400,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its amino acid composition and electron microscopic appearance were similar to those reported earlier for mouse laminin. Carbohydrate analysis revealed 13% carbohydrate consisting of N-acetylglucosamine, galactose, mannose, fucose, sialic acid, and small amounts of N-acetyl galactosamine. The purified rat laminin was immunologically very similar to mouse laminin as recognized by rabbit antibodies but was antigenically distinct when recognized by mouse antibodies.
Authors:
E Engvall; T Krusius; U Wewer; E Ruoslahti
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  222     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  649-56     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Antibodies / analysis
Carbohydrates / analysis
Chemical Phenomena
Chemistry
Glycoproteins / isolation & purification*
Laminin
Membrane Proteins / isolation & purification*
Mesonephroma / analysis*
Mice
Microscopy, Electron
Neoplasm Proteins / isolation & purification*
Neoplasms, Experimental / analysis
Rats
Species Specificity
Grant Support
ID/Acronym/Agency:
AM 30051/AM/NIADDK NIH HHS; CA 28896/CA/NCI NIH HHS; CA 30199/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Antibodies; 0/Carbohydrates; 0/Glycoproteins; 0/Laminin; 0/Membrane Proteins; 0/Neoplasm Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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