Document Detail

Laminin SIKVAV peptide induction of monocyte/macrophage prostaglandin E2 and matrix metalloproteinases.
MedLine Citation:
PMID:  7737965     Owner:  NLM     Status:  MEDLINE    
The laminin-derived synthetic peptide containing the SIKVAV (Ser-Ile-Lys-Val-Ala-Val) amino acid sequence has been previously shown to regulate tumor invasion, metastasis, and angiogenesis. Here, we demonstrate that this peptide also modulates human monocyte responses. Moreover, the monocytic responses elicited by this peptide are influenced by the culture conditions. When elutriated monocytes were cultured on SIKVAV substrate or in suspension with this peptide, the synthesis of prostaglandin E2, interstitial collagenase, and gelatinase B was induced and was further enhanced in the presence of concanavalin A (ConA). However, when monocytes were adhered before adding soluble SIKVAV, the peptide alone failed to induce the production of prostaglandin E2 or matrix metalloproteinases. If adherent monocytes were exposed to SIKVAV in the presence of ConA, this peptide enhanced the ConA induced production of these mediators. In contrast to SIKVAV, the intact laminin molecule failed to influence these monocyte responses. This is the first demonstration that a laminin derived peptide is capable of inducing or enhancing monocyte inflammatory responses that may influence a number of biological activities such as wound healing or excessive connective tissue destruction associated with chronic inflammation.
M L Corcoran; M C Kibbey; H K Kleinman; L M Wahl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-05     Completed Date:  1995-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10365-8     Citation Subset:  IM    
Cellular Immunology Section, NIDR, National Institutes of Health, Bethesda, Maryland 20892-4352, USA.
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MeSH Terms
Amino Acid Sequence
Cell Adhesion
Collagenases / biosynthesis*
Dinoprostone / metabolism*
Laminin / chemistry*
Macrophages / metabolism*
Matrix Metalloproteinase 9
Molecular Sequence Data
Monocytes / metabolism*
Peptide Fragments / pharmacology*
Reg. No./Substance:
0/Laminin; 0/Peptide Fragments; 363-24-6/Dinoprostone; EC 3.4.24.-/Collagenases; EC Metalloproteinase 9

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