Document Detail

Laminar and subcellular heterogeneity of glast and GLT-1 immunoreactivity in the developing postnatal mouse hippocampus.
MedLine Citation:
PMID:  23939750     Owner:  NLM     Status:  Publisher    
Astrocytes express two sodium-coupled transporters, GLAST and GLT-1, which are essential for the maintenance of low extracellular glutamate levels. Here, we performed a comparative analysis of the laminar and subcellular expression profile of GLAST and GLT-1 in the developing postnatal mouse hippocampus using immunohistochemistry and western blotting and employing high-resolution fluorescence microscopy. Astrocytes were identified by co-staining with GFAP or S100ß. In CA1, the density of GFAP-positive cells and GFAP expression rose during the first two weeks after birth, paralleled by a steady increase in GLAST immunoreactivity and protein content. Up-regulation of GLT-1 was completed only at postnatal days (P) P20-25 and thus delayed by about ten days. GLAST staining was highest along stratum pyramidale and was especially prominent in astrocytes at P3-5. GLAST immunoreactivity indicated no preferential localization to a specific cellular compartment. GLT-1 exhibited a laminar expression pattern from P10-15 on, with highest immunoreactivity in the stratum lacunosum-moleculare. At the cellular level, GLT-1 immunoreactivity did not entirely cover astrocyte somata and exhibited clusters at processes. In juvenile animals, discrete clusters of GLT-1 were also detected at perivascular endfeet. From these results, we conclude there is a remarkable subcellular heterogeneity of GLAST and GLT-1 expression in the developing hippocampus. The clustering of GLT-1 at astrocyte endfeet indicates that it might serve a specialized functional role at the blood-brain barrier during formation of the hippocampal network. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
Alexandra E Schreiner; Simone Durry; Tomomi Aida; Martin C Stock; Ulrich Rüther; Kohichi Tanaka; Christine R Rose; Karl W Kafitz
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-8-12
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  -     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc., A Wiley Company.
Institute of Neurobiology, Heinrich Heine University Duesseldorf, Universitaetsstrasse 1, 40225, Duesseldorf, Germany.
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