| A Laminar Flow Electroporation System for Efficient DNA and siRNA Delivery. | |
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MedLine Citation:
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PMID: 21678996 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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By introducing a hydrodynamic mechanism into a microfluidics-based electroporation system, we developed a novel laminar flow electroporation system with high performance. The laminar buffer flow implemented in the system separated the cell suspension flow from the electrodes, thereby excluding many unfavourable effects due to electrode reaction during electroporation, such as hydrolysis, bubble formation, pH change and heating. Compared to conventional microfluidic electroporation systems, these improvements significantly enhanced transfection efficacy and cell viability. Furthermore, successful electrotransfection of plasmid DNA and more importantly, synthetic siRNA, was demonstrated in several hard-to-transfect cell types using this system. |
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Authors:
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Zewen Wei; Deyao Zhao; Xueming Li; Mengxi Wu; Wei Wang; Huang Huang; Xiaoxia Wang; Quan Du; Zicai Liang; Zhihong Li |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-17 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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