| Lamina-specific distribution of Synaptopodin, an actin-associated molecule essential for the spine apparatus, in identified principal cell dendrites of the mouse hippocampus. | |
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MedLine Citation:
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PMID: 15892100 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synaptopodin is an actin-associated molecule found in a subset of telencephalic spines. It is an essential component of the spine apparatus, a Ca(2+)-storing organelle and has been implicated in synaptic plasticity (Deller et al. [2003] Proc Natl Acad Sci U S A 100:10494-10499). In the rodent hippocampus, Synaptopodin is distributed in a characteristic region- and lamina-specific manner. To learn more about the cellular basis underlying this distribution, the regional, laminar, and cellular localization of Synaptopodin and its mRNA were analyzed in mouse hippocampus. First, Synaptopodin puncta densities were quantified after immunofluorescent labeling using confocal microscopy. Second, the dendritic distribution of Synaptopodin-positive puncta was studied using three-dimensional confocal reconstructions of Synaptopodin-immunostained and enhanced green fluorescence protein (EGFP)-labeled principal neurons. Synaptopodin puncta located within dendrites of principal neurons were primarily found in spines (>95%). Analysis of dendritic segments located in different layers revealed lamina-specific differences in the percentage of Synaptopodin-positive spines. Densities ranged between 37% (outer molecular layer) and 14% (stratum oriens; CA1). Finally, synaptopodin mRNA expression was studied using in situ hybridization, laser microdissection, and quantitative reverse transcriptase-polymerase chain reaction. Expression levels were comparable between all regions. These data demonstrate a lamina-specific distribution of Synaptopodin within dendritic segments of identified neurons. Within dendrites, the majority of Synaptopodin-positive puncta were located in spines where they represent spine apparatuses. We conclude, that this organelle is distributed in a region- and layer-specific manner in the mouse hippocampus and suggest that differences in the activity of afferent fiber systems could determine its distribution. |
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Authors:
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Carlos Bas Orth; Andreas Vlachos; Domenico Del Turco; Guido J Burbach; Carola A Haas; Peter Mundel; Guoping Feng; Michael Frotscher; Thomas Deller |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 487 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-05-18 Completed Date: 2005-09-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 227-39 Citation Subset: IM |
Copyright Information:
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Copyright 2005 Wiley-Liss, Inc. |
Affiliation:
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Institute of Clinical Neuroanatomy, J. W. Goethe University, Frankfurt/Main, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dendritic Spines / metabolism* Fluorescent Antibody Technique Hippocampus / cytology, metabolism* Male Mice Mice, Inbred C57BL Microfilament Proteins / genetics, metabolism* Nerve Tissue Proteins / genetics, metabolism* Neuronal Plasticity / physiology RNA, Messenger / analysis Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Microfilament Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Synpo protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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