| Lamin A is not synthesized as a larger precursor polypeptide. | |
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MedLine Citation:
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PMID: 3426582 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolation of rat liver nuclei in the presence of N-ethylmaleimide (NEM) led to the recovery in the final nuclear matrix of a higher molecular weight form of lamin A. The 2 kDa larger form was identified as lamin A by isoelectric point determination, recognition by an anti-lamin A and C monoclonal antibody and peptide mapping using V8 protease and N-chlorosuccinimide. The 2 kDa extension was tentatively localized to the carboxy-terminus of lamin A. Pulse-chase labeling and immunoprecipitation studies using baby hamster kidney cells showed that lysis of the cells in the presence of NEM allowed the recovery of a stable higher molecular weight form of lamin A. We conclude from these results that NEM prevented the degradation of the native form of lamin A previously thought to represent a higher molecular weight transient precursor form. |
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Authors:
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S Lebel; Y Raymond |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 149 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-01-28 Completed Date: 1988-01-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 417-23 Citation Subset: IM |
Affiliation:
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Institut du Cancer de Montréal, Hôpital Notre-Dame, Québec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Ethylmaleimide / pharmacology Lamin Type A Lamins Male Molecular Weight Nuclear Proteins / biosynthesis* Protein Precursors / biosynthesis* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Lamin Type A; 0/Lamins; 0/Nuclear Proteins; 0/Protein Precursors; 128-53-0/Ethylmaleimide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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