Document Detail


Lamellar structure in poly(ala-gly) determined by solid-state NMR and statistical mechanical calculations.
MedLine Citation:
PMID:  17417849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lamellar structure of poly(Ala-Gly) or (AG)n in the solid was examined using 13C solid-state NMR and statistical mechanical approaches. Two doubly labeled versions, [1-13C]Gly14[1-13C]Ala15- and [1-13C]Gly18[1-13C]Ala19 of (AG)15 were examined by two-dimensional (2D) 13C spin diffusion NMR in the solid state. In addition five doubly labeled [15N,13C]-versions of the same peptide, (AG) 15 and 15 versions labeled [3-13C] in each of the successive Ala residues were utilized for REDOR and 13C CP/MAS NMR measurements, respectively. The observed spin diffusion NMR spectra were consistent with a structure containing a combination of distorted beta-turns with a large distribution of the torsion angles and antiparallel beta-sheets. The relative proportion of the distorted beta-turn form was evaluated by examination of 13C CP/MAS NMR spectra of [3-13C]Ala-(AG)15. In addition, REDOR determinations showed five kinds of atomic distances between doubly labeled 13C and 15N nuclei which were also interpreted in terms of a combination of beta-sheets and beta-turns. Our statistical mechanical analysis is in excellent agreement with our Ala Cbeta 13C CP/MAS NMR data strongly suggesting that (AG)15 has a lamellar structure.
Authors:
Tetsuo Asakura; Hirohiko Sato; Fumika Moro; Yasumoto Nakazawa; Akihiro Aoki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-07
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  129     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-25     Completed Date:  2007-07-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5703-9     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan. asakura@cc.tuat.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Indicators and Reagents
Magnetic Resonance Spectroscopy
Molecular Conformation
Peptides / chemistry*
Silk / chemistry*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Peptides; 0/Silk; 27879-92-1/poly(alanylglycine)

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