| Lambda-carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells. | |
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MedLine Citation:
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PMID: 19885621 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown that highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) possess an anti-angiogenetic effect, while high concentrations of lambda-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of lambda-CO on inhibiting cell proliferation. lambda-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, prevention of oxidative stress by N-acetyl-L-cysteine (NAC) could abolish the effect of lambda-CO on these events. Yet, lambda-CO induced a depolarization of mitochondrial transmembrane potential. At 0.8 mg/ml, lambda-CO induced up-regulation of p53 and Bax, down-regulation of Bcl-2 and activation of caspase-9 and -3. These results suggest that exposure to a high concentration of lambda-CO activates the mitochondrial-mediated apoptotic pathway and cell cycle arrest by generation of ROS. |
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Authors:
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Hai-Min Chen; Xiao-Jun Yan; Ting-Yan Mai; Feng Wang; Wei-Feng Xu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 24 ISSN: 1791-244X ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-03 Completed Date: 2010-02-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 801-6 Citation Subset: IM |
Affiliation:
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Ningbo University, Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Zhejiang 315211, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Apoptosis / drug effects*, physiology Apoptosis Regulatory Proteins / metabolism Carrageenan / pharmacology* Caspase 3 / metabolism Caspase 9 / metabolism Cell Cycle / drug effects Cell Nucleus / drug effects Cell Proliferation / drug effects Cells, Cultured Endothelial Cells Flow Cytometry Humans Membrane Potential, Mitochondrial / drug effects Mitochondria / drug effects*, physiology Oxidative Stress / drug effects* Proto-Oncogene Proteins c-bcl-2 / metabolism Reactive Oxygen Species / metabolism* Tumor Suppressor Protein p53 / metabolism Umbilical Veins bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/BAX protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/Reactive Oxygen Species; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 616-91-1/Acetylcysteine; 9000-07-1/Carrageenan; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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