| Lag-phase autophagy in the methylotrophic yeast Pichia pastoris. | |
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MedLine Citation:
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PMID: 19549169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When microbes sense environmental changes, they often temporarily attenuate cell growth to adapt to the new situations, showing a lag phase. In this study, we report that the methylotrophic yeast, Pichia pastoris, induced autophagy during the lag phase after the cells were shifted from glucose to methanol medium. Through the autophagic process at least two proteins, aminopeptidase I precursor and cytosolic aldehyde dehydrogenase, were found to be transported into the vacuole, which was dependent on PpAtg11 and PpAtg17, respectively. Notably, PpAtg1 and PpAtg17 were required for early exit from the lag phase during the methanol adaptation. In accordance, phosphorylation states of elongation initiation factor 2alpha indicated reductions of intracellular amino-acid pools in the atg mutant strains. Together, these data demonstrate the importance of amino acid recycling by autophagy during a cell-remodeling process. |
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Authors:
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Shun-ichi Yamashita; Hiroya Yurimoto; Dai Murakami; Mari Yoshikawa; Masahide Oku; Yasuyoshi Sakai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-22 |
Journal Detail:
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Title: Genes to cells : devoted to molecular & cellular mechanisms Volume: 14 ISSN: 1365-2443 ISO Abbreviation: Genes Cells Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-08 Completed Date: 2009-10-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607379 Medline TA: Genes Cells Country: England |
Other Details:
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Languages: eng Pagination: 861-70 Citation Subset: IM |
Affiliation:
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Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Dehydrogenase
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metabolism Autophagy / physiology* Cytosol / metabolism Fungal Proteins / metabolism Glucose / metabolism Methanol / metabolism Phosphorylation Pichia / genetics, metabolism* Vacuoles / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 50-99-7/Glucose; 67-56-1/Methanol; EC 1.2.1.3/Aldehyde Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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