| Lactoferrin impairs type III secretory system function in enteropathogenic Escherichia coli. | |
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MedLine Citation:
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PMID: 12933858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enteropathogenic Escherichia coli (EPEC) is an important cause of infant diarrhea in developing countries. EPEC uses a type III secretory system to deliver effector proteins into the host cell. These proteins cause the characteristic attaching and effacing lesion on enterocytes. Lactoferrin, a glycoprotein present in human milk, inhibits EPEC adherence to mammalian cells. To determine the effect of lactoferrin on the initial host cell attachment step that is mediated by the type III secretory system, we focused on EPEC-induced actin polymerization in HEp2 cells, on the hemolytic activity, and on measurement of E. coli secreted proteins A, B, and D (EspABD). Lactoferrin blocked EPEC-mediated actin polymerization in HEp2 cells and blocked EPEC-induced hemolysis. The mechanism of this inhibition was lactoferrin-mediated degradation of secreted proteins necessary for bacterial contact and pore formation, particularly EspB. The proteolytic effect of lactoferrin was prevented by serine protease inhibitors. This disruption of the type III secretory system implies that lactoferrin could provide broad cross protection against the enteropathogens that share this mechanism. |
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Authors:
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Theresa J Ochoa; Marita Noguera-Obenza; Frank Ebel; Carlos A Guzman; Henry F Gomez; Thomas G Cleary |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 71 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-08-22 Completed Date: 2003-09-29 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 5149-55 Citation Subset: IM |
Affiliation:
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Division of Pediatric Infectious Diseases, University of Texas-Houston Medical School, Houston, Texas 77040, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Bacterial Adhesion / drug effects Bacterial Outer Membrane Proteins / physiology Bacterial Proteins / physiology Cell Line Diarrhea / prevention & control Escherichia coli / drug effects*, growth & development, pathogenicity*, physiology Escherichia coli Infections / prevention & control Escherichia coli Proteins / physiology Hemolysis / drug effects Humans Infant Lactoferrin / pharmacology* Recombinant Proteins / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P01-HD 13021-25/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/EaeB protein, E coli; 0/Escherichia coli Proteins; 0/EspA protein, E coli; 0/EspD protein, E coli; 0/Recombinant Proteins; EC 3.4.21.-/Lactoferrin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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