Document Detail

Lactoferrin impairs type III secretory system function in enteropathogenic Escherichia coli.
MedLine Citation:
PMID:  12933858     Owner:  NLM     Status:  MEDLINE    
Enteropathogenic Escherichia coli (EPEC) is an important cause of infant diarrhea in developing countries. EPEC uses a type III secretory system to deliver effector proteins into the host cell. These proteins cause the characteristic attaching and effacing lesion on enterocytes. Lactoferrin, a glycoprotein present in human milk, inhibits EPEC adherence to mammalian cells. To determine the effect of lactoferrin on the initial host cell attachment step that is mediated by the type III secretory system, we focused on EPEC-induced actin polymerization in HEp2 cells, on the hemolytic activity, and on measurement of E. coli secreted proteins A, B, and D (EspABD). Lactoferrin blocked EPEC-mediated actin polymerization in HEp2 cells and blocked EPEC-induced hemolysis. The mechanism of this inhibition was lactoferrin-mediated degradation of secreted proteins necessary for bacterial contact and pore formation, particularly EspB. The proteolytic effect of lactoferrin was prevented by serine protease inhibitors. This disruption of the type III secretory system implies that lactoferrin could provide broad cross protection against the enteropathogens that share this mechanism.
Theresa J Ochoa; Marita Noguera-Obenza; Frank Ebel; Carlos A Guzman; Henry F Gomez; Thomas G Cleary
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  71     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-22     Completed Date:  2003-09-29     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5149-55     Citation Subset:  IM    
Division of Pediatric Infectious Diseases, University of Texas-Houston Medical School, Houston, Texas 77040, USA.
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MeSH Terms
Actins / metabolism
Bacterial Adhesion / drug effects
Bacterial Outer Membrane Proteins / physiology
Bacterial Proteins / physiology
Cell Line
Diarrhea / prevention & control
Escherichia coli / drug effects*,  growth & development,  pathogenicity*,  physiology
Escherichia coli Infections / prevention & control
Escherichia coli Proteins / physiology
Hemolysis / drug effects
Lactoferrin / pharmacology*
Recombinant Proteins / pharmacology
Grant Support
P01-HD 13021-25/HD/NICHD NIH HHS
Reg. No./Substance:
0/Actins; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/EaeB protein, E coli; 0/Escherichia coli Proteins; 0/EspA protein, E coli; 0/EspD protein, E coli; 0/Recombinant Proteins; EC 3.4.21.-/Lactoferrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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