| Lactococcus lactis: high-level expression of tetanus toxin fragment C and protection against lethal challenge. | |
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MedLine Citation:
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PMID: 8361360 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expression of foreign antigens (and other proteins) in L. lactis. The system utilizes the fast-acting T7 RNA polymerase to transcribe target genes, and provides the first example of the successful use of this polymerase in a Gram-positive bacterium. When the performance of the expression system was characterized using tetanus toxin fragment C (TTFC) up to 22% of soluble cell protein was routinely obtained as TTFC. Mice immunized subcutaneously with L. lactis expressing TTFC were protected from lethal challenge with tetanus toxin. These results show for the first time that L. lactis is able to express substantial quantities of a heterologous protein antigen and that this organism can present this antigen to the immune system in an immunogenic form. |
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Authors:
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J M Wells; P W Wilson; P M Norton; M J Gasson; R W Le Page |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular microbiology Volume: 8 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-09-30 Completed Date: 1993-09-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1155-62 Citation Subset: IM |
Affiliation:
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University of Cambridge, Department of Pathology, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cloning, Molecular DNA-Directed RNA Polymerases / metabolism Gene Expression Regulation, Bacterial Genetic Vectors Lactococcus lactis / genetics* Mice Mice, Inbred CBA / immunology Molecular Sequence Data Nucleic Acid Conformation Peptide Fragments / biosynthesis*, genetics, immunology Recombinant Fusion Proteins / biosynthesis*, genetics, immunology Tetanus / prevention & control Tetanus Toxin / biosynthesis*, genetics, immunology Tetanus Toxoid* Vaccines, Synthetic Viral Proteins |
| Chemical | |
Reg. No./Substance:
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0/Peptide Fragments; 0/Recombinant Fusion Proteins; 0/Tetanus Toxin; 0/Tetanus Toxoid; 0/Vaccines, Synthetic; 0/Viral Proteins; 0/tetanus toxin fragment C; EC 2.7.7.-/bacteriophage T7 RNA polymerase; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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