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Lactobacillus plantarum CS24.2 prevents E. coli adhesion to HT-29 cells and also down-regulates enteropathogen induced TNF-α and IL-8 expression.
MedLine Citation:
PMID:  23409866     Owner:  NLM     Status:  Publisher    
The aim of the present study was to evaluate the potential of Lactobacillus plantarum CS24.2 to antagonize Escherichia coli adhesion and modulate the expression of inflammatory molecules by HT-29 cells response to the E. coli adhesion. This was carried out under different adhesion conditions and compared with the response seen in case of L. rhamnosus GG. The competitive adhesion, adhesion inhibition and displacement assays were carried out for adhesion of lactobacilli (L. rhamnosus GG and L. plantarum CS24.2) and E. coli O26:H11 to HT-29 cells. Both the lactobacilli significantly reduced E. coli adhesion to HT-29 cells (p < 0.05). The ability of lactobacilli to modulate TNF-α and IL-8 expression was analyzed in HT-29 cells stimulated with E. coli using qRT-PCR. L. plantarum CS24.2 significantly down regulated the expression of both the genes induced by E. coli in HT-29 cells at 6 h as well as 24 h which was more significant then that of L. rhamnosus GG. The results suggest that L. plantarum CS24.2 has the ability to effectively inhibit pathogen adhesion which was comparable with an established probiotic strain L. rhamnosus GG. It could also attenuate TNF-α and IL-8 expression in HT-29 cells stimulated with E. coli.
Akhilesh S Dhanani; Tamishraha Bagchi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-15
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Societies and Blackwell Publishing Asia Pty Ltd.
Department of Microbiology and Biotechnology Centre, Faculty of Science, The M.S. University of Baroda, Vadodara-390002, Gujarat, India.
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