| Lactobacillus fermentum ACA-DC 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (TNBS)-induced colitis and Salmonella infection in murine models. | |
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MedLine Citation:
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PMID: 18077037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lactobacillus fermentum ACA-DC 179, Lactobacillus plantarum ACA-DC 287 and Streptococcus macedonicus ACA-DC 198 were studied for their probiotic potential. Firstly, strains were screened for antimicrobial activity towards a broad range of target strains, including lactic acid bacteria, food spoilage and pathogenic bacteria. L. fermentum ACA-DC 179 was active against five streptococci, including the two pathogenic strains Streptococcus oralis LMG 14532T and Streptococcus pneumoniae LMG 14545T. S. macedonicus ACA-DC 198 was active against the majority of the strains tested, including not only lactic acid bacteria but also many food spoilage or pathogenic species. The three potential probiotic strains were found to survive variably at pH 2.5 and were unaffected by bile salts. Only S. macedonicus ACA-DC 198 exhibited bile salt hydrolase activity, while none of the strains was haemolytic. Moreover, strains exhibited variable susceptibility towards commonly used antibiotics. L. plantarum ACA-DC 287 and S. macedonicus ACA-DC 198 induced the secretion of the pro-inflammatory cytokines IL-12, IFN-gamma and TNF-alpha by human peripheral blood mononuclear cells. Also elevated levels of the anti-inflammatory IL-10 were observed with L. fermentum ACA-DC 179. This strain consequently was found to significantly reduce colitis in a TNBS-induced colitis mouse model. Furthermore, L. fermentum ACA-DC 179 was successfully applied in an experimental Salmonella-infection mouse model. To conclude, strain L. fermentum ACA-DC 179 possesses desirable probiotic properties, such as antimicrobial activity and immunomodulation in vitro, which were confirmed in vivo by the use of animal models. |
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Authors:
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Georgia Zoumpopoulou; Benoit Foligne; Kostas Christodoulou; Corinne Grangette; Bruno Pot; Effie Tsakalidou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-06 |
Journal Detail:
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Title: International journal of food microbiology Volume: 121 ISSN: 0168-1605 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-24 Completed Date: 2008-04-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 18-26 Citation Subset: IM |
Affiliation:
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Laboratory of Dairy Research, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiosis* Cell-Free System Colitis / chemically induced, microbiology, prevention & control* Colony Count, Microbial Cytokines / biosynthesis Disease Models, Animal Female Food Microbiology* Humans Hydrogen-Ion Concentration Lactobacillus fermentum / physiology* Lactobacillus plantarum / growth & development, physiology Leukocytes, Mononuclear Mice Mice, Inbred BALB C Probiotics* Salmonella Infections / microbiology, prevention & control* Streptococcus / growth & development, physiology Trinitrobenzenesulfonic Acid / toxicity |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 2508-19-2/Trinitrobenzenesulfonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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