| Lactobacillus acidophilus and Lactobacillus reuteri modulate cytokine responses in gnotobiotic pigs infected with human rotavirus. | |
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MedLine Citation:
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PMID: 22348907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Probiotic lactic acid bacteria (LAB) have been shown to alleviate inflammation, enhance the immunogenicity of rotavirus vaccines, or reduce the severity of rotavirus diarrhoea. Although the mechanisms are not clear, the differential Th1/Th2/Th3-driving capacities and modulating effects on cytokine production of different LAB strains may be the key. Our goal was to delineate the influence of combining two probiotic strains of Lactobacillus acidophilus and Lactobacillus reuteri on the development of cytokine responses in neonatal gnotobiotic pigs infected with human rotavirus (HRV). We demonstrated that HRV alone, or HRV plus LAB, but not LAB alone, initiated serum cytokine responses, as indicated by significantly higher concentrations of IFN-α, IFN-γ, IL-12, and IL-10 at postinoculation day (PID) 2 in the HRV only and LAB+HRV+ pigs compared to LAB only and LAB-HRV- pigs. Peak cytokine responses coincided with the peak of HRV replication. LAB further enhanced the Th1 and Th2 cytokine responses to HRV infection as indicated by significantly higher concentrations of IL-12, IFN-γ, IL-4 and IL-10 in the LAB+HRV+ pigs compared to the LAB-HRV+ pigs. The LAB+HRV+ pigs maintained relatively constant concentrations of TGF-β compared to the HRV only group which had a significant increase at PID 2 and decrease at PID 7, suggesting a regulatory role of LAB in maintaining gut homeostasis. At PID 28, cytokine secreting cell (CSC) responses, measured by ELISpot, showed increased Th1 (IL-12, IFN-γ) CSC numbers in the LAB+HRV+ and LAB-HRV+ groups compared to LAB only and LAB-HRV- pigs, with significantly increased IL-12 CSCs in spleen and PBMCs and IFN-γ CSCs in spleen of the LAB+HRV+ group. Thus, HRV infection alone, but not LAB alone was effective in inducing cytokine responses but LAB significantly enhanced both Th1 and Th2 cytokines in HRV-infected pigs. LAB may also help to maintain immunological homeostasis during HRV infection by regulating TGF-β production. |
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Authors:
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M S P Azevedo; W Zhang; K Wen; A M Gonzalez; L J Saif; A E Yousef; L Yuan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Beneficial microbes Volume: 3 ISSN: 1876-2891 ISO Abbreviation: Benef Microbes Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-02-21 Completed Date: 2012-06-14 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 101507616 Medline TA: Benef Microbes Country: Netherlands |
Other Details:
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Languages: eng Pagination: 33-42 Citation Subset: IM |
Affiliation:
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US Food and Drug Administration, National Center for Toxicological Research, Division of Microbiology, Jefferson, AR 72079, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytokines / immunology* Diarrhea / immunology, virology Enzyme-Linked Immunospot Assay Germ-Free Life Intestines / immunology, microbiology Lactobacillus acidophilus / immunology* Lactobacillus reuteri / immunology* Probiotics / administration & dosage Rotavirus / immunology*, pathogenicity Rotavirus Infections / immunology*, virology Spleen / immunology, virology Swine / immunology*, microbiology, virology Th1 Cells / immunology Th2 Cells / immunology |
| Grant Support | |
ID/Acronym/Agency:
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1R21AT002524/AT/NCCAM NIH HHS; R01 AI033561-10/AI/NIAID NIH HHS; R01AI033561/AI/NIAID NIH HHS; R21 AT002524-01/AT/NCCAM NIH HHS; R21 AT002524-02/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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