Document Detail


Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: a response to ethanol stress?
MedLine Citation:
PMID:  11984636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is known that ethanol strongly interferes with the development and activity of lactic acid bacteria in wine. In this work, it was observed that membrane composition was dependent of ethanol concentration and cell physiological state. The protein electrophoretic profile was modified in the membranes of Oenococcus oeni cultured in presence of 8 and 10% ethanol. Concerning the membrane lipid composition, it was observed that O. oeni maintained a high level of phospholipid biosynthesis via the relative increased biosynthesis of phosphoethanolamine and sphingomyelin in presence of ethanol. On the other hand, ethanol induced an increase in the membrane lactobacillic acid percentage at the expense of cis-vaccenic acid. This increased synthesis of lactobacillic acid appears as the more significant change induced by ethanol in O. oeni membrane. The increase of lactobacillic acid in the membrane of O. oeni clearly appears as a factor that provides protection against the toxic effect of ethanol, balancing the increase of membrane fluidity normally attributed to ethanol. The results presented in this paper constitute evidence that lactobacillic acid may have a part in the survival and or adaptive mechanisms developed by O. oeni under culture adverse conditions, allowing these bacteria to maintain their activity in the presence of ethanol, namely performing malolactic fermentation in wine.
Authors:
H Teixeira; M G Gonçalves; N Rozès; A Ramos; M V San Romão
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-01-23
Journal Detail:
Title:  Microbial ecology     Volume:  43     ISSN:  0095-3628     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-07-02     Completed Date:  2002-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  146-53     Citation Subset:  IM    
Affiliation:
Instituto de Biologia Experimental and Tecnologica, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, 2781-901 Oeiras, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Cell Membrane / physiology
Central Nervous System Depressants / pharmacology*
Ethanol / pharmacology*
Fatty Acids / analysis*
Fermentation
Gram-Positive Cocci / physiology*
Leuconostoc / physiology
Population Dynamics
Survival Analysis
Wine
Chemical
Reg. No./Substance:
0/Central Nervous System Depressants; 0/Fatty Acids; 503-06-0/phytomonic acid; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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