Document Detail


Lactobacilli, bifidobacteria and E. coli nissle induce pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells.
MedLine Citation:
PMID:  17009396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate whether the stimulation of peripheral blood mononuclear cells (PBMNC) with the cell debris and cell extraction of different probiotic strains is similar or species specific.
METHODS: Three strains of bifidobacteria, 4 strains of lactobacilli, and E. coli nissle were sonicated and centrifuged in order to divide them into cell extract and cell debris. PBMNC were separated by density gradient and incubated for 36 h with either the cell debris or the cell extract of single strains of probiotic bacteria in doses from 10(2) to 10(8) CFU/mL. Cell supernatants were taken and interleukin (IL)-10, IL-1beta, and tumor necrosis factor (TNF)-alpha were determined by ELISA.
RESULTS: Depending on the species super-family, the strains had different stimulation patterns. Except for both L. casei strains, the cell extract of bifidobacteria and lactobacilli had less stimulating capacity than cell debris, whereas the cell extract of E. coli nissle had similar stimulating properties to that of the cell debris of the strain and significantly more stimulating capacity than that of bifidobacteria and lactobacilli. The cell debris of bifidobacteria stimulated more cytokine release than the cell debris of lactobacilli. The cell debris of lactobacilli did not have a stimulating capacity when lower concentrations were used. Neither cell extraction nor cell debris had an inhibitory effect on the production of the tested cytokines by stimulated PBMNC.
CONCLUSION: The incubation of probiotic strains, which have been used in clinical trials for inflammatory diseases, with immunocompetent cells leads to different species specific reactions. High IL-10 response to cell debris of bifidobacteria and E. coli nissle can be found. This corresponds to positive effects of bifidobacteria and E. coli nissle in clinical trials for inflammatory bowel disease compared to negative outcomes obtained with lactobacilli.
Authors:
Ulf Helwig; Karen M Lammers; Fernando Rizzello; Patricia Brigidi; Verena Rohleder; Elisabetta Caramelli; Paolo Gionchetti; Juergen Schrezenmeir; Ulrich R Foelsch; Stefan Schreiber; Massimo Campieri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  12     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-29     Completed Date:  2006-11-30     Revised Date:  2014-08-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  5978-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Bifidobacterium / physiology*
Cell Extracts / pharmacology
Cells, Cultured
Cytokines / metabolism*
Escherichia coli / physiology*
Female
Humans
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Lactobacillus / physiology*
Leukocytes, Mononuclear / cytology,  drug effects,  metabolism*,  microbiology
Male
Middle Aged
Probiotics / pharmacology
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Cytokines; 0/Interleukin-1beta; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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