Document Detail

Lacto-N-tetraose, fucosylation, and secretor status are highly variable in human milk oligosaccharides from women delivering preterm.
MedLine Citation:
PMID:  22900748     Owner:  NLM     Status:  MEDLINE    
Breast milk is the ideal nutrition for term infants but must be supplemented to provide adequate growth for most premature infants. Human milk oligosaccharides (HMOs) are remarkably abundant and diverse in breast milk and yet provide no nutritive value to the infant. HMOs appear to have at least two major functions: prebiotic activity (stimulation of the growth of commensal bacteria in the gut) and protection against pathogens. Investigations of HMOs in milk from women delivering preterm have been limited. We present the first detailed mass spectrometric analysis of the fucosylation and sialylation in HMOs in serial specimens of milk from 15 women delivering preterm and 7 women delivering at term using nanohigh performance liquid chromatography chip/time-of-flight mass spectrometry. A mixed-effects model with Levene's test was used for the statistical analyses. We find that lacto-N-tetraose, a core HMO, is both more abundant and more highly variable in the milk of women delivering preterm. Furthermore, fucosylation in preterm milk is not as well regulated as in term milk, resulting in higher within and between mother variation in women delivering preterm vs term. Of particular clinical interest, the α1,2-linked fucosylated oligosaccharide 2'-fucosyllactose, an indicator of secretor status, is not consistently present across lactation of several mothers that delivered preterm. The immaturity of HMO production does not appear to resolve over the time of lactation and may have relevance to the susceptibility of premature infants to necrotizing enterocolitis, late onset sepsis, and related neurodevelopmental impairments.
Maria Lorna A De Leoz; Stephanie C Gaerlan; John S Strum; Lauren M Dimapasoc; Majid Mirmiran; Daniel J Tancredi; Jennifer T Smilowitz; Karen M Kalanetra; David A Mills; J Bruce German; Carlito B Lebrilla; Mark A Underwood
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-28
Journal Detail:
Title:  Journal of proteome research     Volume:  11     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-07     Completed Date:  2013-03-28     Revised Date:  2013-09-16    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4662-72     Citation Subset:  IM    
Department of Chemistry, University of California, Davis, California 95616, USA.
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MeSH Terms
Birth Weight
Chromatography, High Pressure Liquid
Milk, Human / chemistry*,  metabolism
N-Acetylneuraminic Acid / metabolism
Oligosaccharides / analysis*,  metabolism
Premature Birth / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Trisaccharides / analysis*,  metabolism
Grant Support
Reg. No./Substance:
0/Oligosaccharides; 0/Trisaccharides; 13007-32-4/lacto-N-neotetraose; 131-48-6/N-Acetylneuraminic Acid; 41263-94-9/2'-fucosyllactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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