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Lactic acid production in Saccharomyces cerevisiae is modulated by the expression of the monocarboxylate transporters Jen1 and Ady2.
MedLine Citation:
PMID:  22260735     Owner:  NLM     Status:  Publisher    
In this work we aimed at manipulating the metabolism of Saccharomyces cerevisiae to produce lactic acid and search for the potential influence of the acid transport across plasma membrane in this process. S. cerevisiae W303-1A is able to use L-lactic acid but its production in our laboratory conditions has never been detected. When L-LDH gene from Lactobacillus casei was expressed in S. cerevisiae W303-1A and in the isogenic mutants jen1∆, ady2∆ and jen1∆ ady2∆, all strains were able to produce lactic acid, but higher titers were achieved in the mutant strains. In strains constitutively expressing both LDH and JEN1 or ADY2 a higher outer lactic acid concentration was found when glucose was present in the medium, but when glucose was exhausted, its consumption was more pronounced. These results demonstrate that monocarboxylates permeases expression influences lactic acid production. Ady2 has been previously characterized as an acetate permease but our results demonstrated its additional role in lactate uptake. Overall we demonstrate that monocarboxylate transporters Jen1 and Ady2 are modulators of lactic acid production and may well be used to manipulate lactic acid export in yeast cells.
António Pacheco; Gabriel Talaia; Joana Sá-Pessoa; Daniela Bessa; Maria José Gonçalves; Roxana Moreira; Sandra Paiva; Margarida Casal; Odília Queirós
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-19
Journal Detail:
Title:  FEMS yeast research     Volume:  -     ISSN:  1567-1364     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Centro de Investigação em Ciências da Saúde (CICS), Instituto Superior de Ciências da Saúde-Norte / CESPU, Rua Central de Gandra, 1317, 4585-116, Gandra PRD, Portugal.
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