| Lactation stage-dependent changes of lymphocyte subpopulations in mammary secretions: inversion of CD4+/CD8+ T cell ratios at parturition. | |
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MedLine Citation:
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PMID: 9196796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: Determination of lactation stage-dependent changes in levels of lymphocyte subpopulations in milk. METHOD: Flow cytometric assay was used to identify and assay lymphocyte subpopulations in bovine milk at different stages of lactation. RESULTS: Lymphocyte subpopulations in mammary secretions of dairy cows change during the lactation cycle. In involuting glands (dry gland), approximately 80-90% of lymphocytes were CD2+ T cells. The proportion of CD2+ T cells, however, decreased to approximately 50% at the colostral stage an fluctuated between 50 to 60% in normal (mature) milk. Throughout the lactation stages, less than 5% were B cells as identified by the monoclonal antibodies against CD21 and MHC class II antigens. Subset analysis showed, however, that the proportion of CD5+ T cells decreased from 90% in involuting gland secretions to 75% in colostrum (peripartum stage), and to approximately 40-50% in the normal (mature) milk, CD4+ T cells constituted between 45 to 55% of lymphocytes in the dry gland secretion but decreased drastically at parturition and maintained at the level below 20% throughout normal lactation. In contrast, the proportion of CD8+ T cells in the dry gland secretion was low, between 30 to 40%, but increased steadily, in an inversely-related manner with that of CD4+ T cells, to approximately 40-50% at parturition and maintained at approximately 30-40% during the normal lactation stage thereafter. Two-color immunofluorescence study revealed further that practically all of the CD8+ cells in dry gland secretions were CD2+, and approximately 40% of them were CD5-. Throughout the lactation cycle, WC1+ gamma delta T cells comprised only 2 to 5% of lymphocytes in mammary secretions. CONCLUSIONS: T lymphocyte subpopulations change dynamically during stages of the lactation cycle. The selective migration of T lymphocyte subpopulations to and from the mammary gland, and their functional roles in the immune competence and regulation of the dam and sucklings remain to be elucidated. |
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Authors:
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T J Yang; I A Ayoub; M J Rewinski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 37 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 1997 May |
Date Detail:
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Created Date: 1997-07-24 Completed Date: 1997-07-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 378-83 Citation Subset: IM; X |
Affiliation:
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Department of Pathobiology, University of Connecticut, Storrs 06269-3089, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD2 / immunology Antigens, CD5 / immunology B-Lymphocytes / immunology CD4-CD8 Ratio* Cattle Female Flow Cytometry Fluorescent Antibody Technique, Indirect Histocompatibility Antigens Class II / immunology Lactation / immunology* Lymphocyte Count Lymphocyte Subsets / immunology* Milk / immunology* Receptors, Antigen, T-Cell, gamma-delta / immunology Receptors, Complement 3d / immunology T-Lymphocytes / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD2; 0/Antigens, CD5; 0/Histocompatibility Antigens Class II; 0/Receptors, Antigen, T-Cell, gamma-delta; 0/Receptors, Complement 3d |
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