| Lactate transport in red blood cells by monocarboxylate transporters. | |
| | |
MedLine Citation:
|
PMID: 12405750 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The lactate transport activity of red blood cells (RBC) varies widely among different species; in equine RBC, the activity of the main lactate carrier, H+-monocarboxylate co-transporter (MCT), is distributed bimodally. The influence of lactate transport activity is measurable in vivo; after maximal exercise, the RBC lactate concentration in horses with high (HT) lactate transport activity is higher than in those with low (LT) activity. To study the expression of MCT in HT and LT horses, blood samples were taken from 10 horses at rest and after submaximal exercise. Blood and plasma lactate concentrations, lactate and pyruvate transport activities and the amounts of MCT1, MCT2 and MCT4 were measured. After exercise, RBC lactate concentration was higher in HT (n = 5) than in LT (n = 5) horses. At lactate concentrations of 0.25-30 mmol/l and at a pyruvate concentration of 1 mmol/l, transport activity was higher in HT horses. At a lactate concentration of 0.1 mmol/l, transport was similar. In Western blots, the signals for MCT1 and MCT2 were similar in both groups. The amount of CD147, a chaperone necessary for the activity of MCT1, was lower in LT horses. We suggest that MCT2 transports lactate at low concentrations, while MCT1 is needed at higher concentrations. MCT1 may be less active in LT horses and, therefore, during exercise their capacity to take up lactate is low. Further studies are needed to show whether the differences in lactate influx in RBC affect the function of erythrocytes or the performance capacity of horses. |
| | |
Authors:
|
N M Koho; L K Väihkönen; A R Pösö |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Equine veterinary journal. Supplement Volume: - ISSN: - ISO Abbreviation: Equine Vet J Suppl Publication Date: 2002 Sep |
Date Detail:
|
Created Date: 2002-10-30 Completed Date: 2003-02-26 Revised Date: 2007-05-18 |
Medline Journal Info:
|
Nlm Unique ID: 9614088 Medline TA: Equine Vet J Suppl Country: England |
Other Details:
|
Languages: eng Pagination: 555-9 Citation Subset: IM |
Affiliation:
|
Department of Basic Veterinary Sciences, University of Helsinki, Finland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Erythrocytes / metabolism* Exercise Test / veterinary Female Horses / blood* Lactates / blood* Male Monocarboxylic Acid Transporters / physiology* Physical Conditioning, Animal / physiology Pyruvates / blood |
| Chemical | |
Reg. No./Substance:
|
0/Lactates; 0/Monocarboxylic Acid Transporters; 0/Pyruvates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of the i-STAT hand-held chemical analyser during treadmill and endurance exercise.
Next Document: Carbonic anhydrase activity and sweat gland morphology in trained and untrained Standardbred trotter...