Document Detail

Lactate production in isolated segments of the rat nephron.
MedLine Citation:
PMID:  3985159     Owner:  NLM     Status:  MEDLINE    
Lactate production was measured directly in individual segments of the rat nephron. Tubules were dissected and then incubated in vitro with glucose as the only metabolic substrate. Each segment was incubated with and without antimycin A, an inhibitor of oxidative metabolism. Proximal tubules produced no lactate with or without antimycin A. The distal segments all produced lactate. The rate of lactate production without antimycin A ranged from 0.4 to 0.9 pmol X min-1 X mm-1 in all distal segments except one, the inner medullary collecting duct, which produced lactate at the significantly higher rate of 2.8 pmol X min-1 X mm-1. Antimycin A increased lactate production significantly in all of the distal segments. The increase was largest in medullary thick ascending limbs (1,400%) and cortical (798%) and outer medullary collecting ducts (357%). Increments were smaller in cortical thick ascending limbs (98%) and distal convoluted tubules (98%) and least in the inner medullary collecting ducts (28%). We conclude that lactate production occurs only in distal segments of the nephron and that under anoxic conditions significant amounts of ATP are produced by anaerobic glycolysis in these segments.
S Bagnasco; D Good; R Balaban; M Burg
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  248     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-05-14     Completed Date:  1985-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F522-6     Citation Subset:  IM    
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MeSH Terms
Antimycin A / pharmacology
Iodoacetates / diagnostic use
Iodoacetic Acid
Kidney Cortex
Kidney Medulla
Kidney Tubules, Distal / metabolism
Kidney Tubules, Proximal / metabolism
Lactates / biosynthesis*
Loop of Henle / metabolism
Nephrons / metabolism*
Time Factors
Tissue Distribution
Reg. No./Substance:
0/Iodoacetates; 0/Lactates; 64-69-7/Iodoacetic Acid; 642-15-9/Antimycin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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