Document Detail


Lactate influx into red blood cells of athletic and nonathletic species.
MedLine Citation:
PMID:  7771571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transport of lactate across the erythrocyte membrane proceeds by three distinct pathways: 1) nonionic diffusion of lactic acid, 2) inorganic anion exchange (band 3), and 3) a monocarboxylate-specific (MC) carrier mechanism. This study determined the contributions of these three pathways in the red blood cells (RBCs) of "athletic" and "nonathletic" species. Blood samples were obtained from four male animals of each species: 1) Canis familiaris (dogs), 2) Capra hircus (goats), 3) Equus caballus (horses), and 4) Bos taurus (cattle). Contribution of each pathway to total lactate influx was determined by measuring L-[14C]lactate influx into lactate-depleted control RBCs, p-chloromercuribenzenesulfonic acid (PCMBS)-treated (1 mM) RBCs, and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-treated (0.2 mM) RBCs at three lactate concentrations ([La] values 1.6, 8.1, and 41 mM). PCMBS blocked MC transport and DIDS blocked the band 3 pathway. Lactate influx into the RBCs of the athletic species was 4-160 times faster (P < 0.05) than influx into the RBCs of the nonathletic species at 8.1 and 41 mM [La] values. Nonionic diffusion was greater in the RBCs of nonathletic animals (approximately 7-25%) than in the RBCs of athletic animals (approximately 4%). A significantly higher percentage of the total lactate influx occurred via the band 3 system in the RBCs from the nonathletic animals (approximately 56-83%) vs. the RBCs from the athletic animals (approximately 6-7%) at all [La] values.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M S Skelton; D E Kremer; E W Smith; L B Gladden
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  268     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-07-06     Completed Date:  1995-07-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R1121-8     Citation Subset:  IM    
Affiliation:
Department of Health & Human Performance, Auburn University, Alabama 36849-5323, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Carboxylic Acids / blood
Cattle
Diffusion
Dogs
Erythrocytes / metabolism*
Goats
Horses
Kinetics
Lactates / blood*
Lactic Acid
Male
Species Specificity
Grant Support
ID/Acronym/Agency:
1RO1AR-40342/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Carboxylic Acids; 0/Lactates; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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