Document Detail


Lactate and glycerol released to the intestinal lumen reflect mucosal injury and permeability changes caused by strangulation obstruction.
MedLine Citation:
PMID:  17622777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The present study evaluates whether microdialysis of glycerol and lactate reflects mucosal injury and permeability changes after strangulation obstruction of the pig small intestine. METHODS: Strangulation obstruction was induced by tightening a rubber band around a small bowel loop until its venous pressure increased to a level just below diastolic aortic pressure (partial strangulation), or further until cessation of flow in the main feeding artery (total strangulation). Mucosal injury and permeability of marker molecules from blood to lumen and vice versa was compared to release of glycerol and lactate to the intestinal lumen. RESULTS: Mucosal injury, hyperpermeability, and release of glycerol were more pronounced after total than after partial strangulation. In animals with partial strangulation there was a complete restitution of the surface epithelium, and luminal glycerol and lumen-to-blood permeability of polyethylene glycol 4000 remained low. Such animals showed a sustained elevation of lactate and blood-to-lumen permeability of fluorescein isothiocyanate dextran after 2 h of partial strangulation, but a decline to baseline levels of these parameters in animals with 1 h partial strangulation. CONCLUSION: Microdialysis of lactate and glycerol in the intestinal lumen may be used to assess structural and functional changes of the intestinal mucosa after strangulation obstruction.
Authors:
I S Juel; E Solligård; E Skogvoll; P Aadahl; J E Grønbech
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-06
Journal Detail:
Title:  European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes     Volume:  39     ISSN:  1421-9921     ISO Abbreviation:  Eur Surg Res     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-10-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0174752     Medline TA:  Eur Surg Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  340-9     Citation Subset:  IM    
Copyright Information:
(c) 2007 S. Karger AG, Basel.
Affiliation:
Department of Surgery, St. Olav University Hospital, Trondheim, Norway. ingebjorg.juel@ntnu.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane Permeability / physiology
Disease Models, Animal
Glycerol / metabolism*
Intestinal Mucosa / blood supply,  pathology,  secretion*
Intestinal Obstruction / metabolism*,  physiopathology
Intestine, Small
Ischemia / metabolism*
Lactic Acid / biosynthesis*
Male
Microdialysis
Reperfusion Injury / physiopathology
Swine
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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