| Lactate formation from [U-13C]aspartate in cultured astrocytes: compartmentation of pyruvate metabolism. | |
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MedLine Citation:
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PMID: 9453229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metabolism of [U-13C]aspartate in cultured astrocytes and the effects of inhibitors of malic enzyme and phosphoenolpyruvate carboxykinase (hydroxymalonate and 3-mercaptopicolinic acid, respectively) were studied using 13C nuclear magnetic resonance (NMR) spectroscopy. The labelling of glutamate and glutamine showed entry of aspartate into the tricarboxylic acid (TCA) cycle after conversion to oxaloacetate. Production of [U-13C]pyruvate from [U-13C]aspartate was revealed by the presence of [U-13C]lactate in incubation media. Furthermore, labelling patterns in C-2 and C-3 in intracellular aspartate showed entry of [1,2-13C]acetyl-CoA into the TCA cycle; evidence for pyruvate-recycling. No reduction in [U-13C]lactate was observed in the presence of either enzyme inhibitor. However, 3-mercaptopicolinic acid reduced incorporation of labelled acetyl-CoA into TCA cycle intermediates, indicating compartmentation of pyruvate production in astrocytes. |
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Authors:
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I J Bakken; L R White; J Aasly; G Unsgård; U Sonnewald |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 237 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1998-03-05 Completed Date: 1998-03-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 117-20 Citation Subset: IM |
Affiliation:
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Department of Physics, Norwegian University of Science and Technology (NTNU), Trondheim. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid / metabolism* Astrocytes / enzymology, metabolism* Cells, Cultured Enzyme Inhibitors / pharmacology Immunohistochemistry Lactic Acid / biosynthesis* Magnetic Resonance Spectroscopy Mice Phosphoenolpyruvate Carboxykinase (ATP) / antagonists & inhibitors, metabolism Pyruvic Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 56-84-8/Aspartic Acid; EC 4.1.1.49/Phosphoenolpyruvate Carboxykinase (ATP) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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