Document Detail


Lactate extraction during net lactate release in legs of humans during exercise.
MedLine Citation:
PMID:  3084443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactate metabolism was studied in six normal males using a primed continuous infusion of lactate tracer during continuous graded supine cycle ergometer exercise. Subjects exercised at 49, 98, 147, and 196 W for 6 min at each work load. Blood was sampled from the brachial artery, the iliac vein, and the brachial vein. Arteriovenous differences were determined for chemical lactate concentration and L-[1-14C]-lactate. Tracer-measured lactate extraction was determined from the decrease in lactate radioactivity per volume of blood perfusing the tissue bed. Net lactate release was determined from the change in lactate concentration across the tissue bed. Total lactate release was taken as the sum of tracer-measured lactate extraction and net (chemical) release. At rest the arms and legs showed tracer-measured lactate extraction, as determined from the isotope extraction, despite net chemical release. Exercise elicited an increase in both net lactate release and tracer-measured lactate extraction by the legs. For the legs the total lactate release (net lactate release + tracer-measured lactate extraction) was roughly equal to twice the net lactate release under all conditions. The tracer-measured lactate extraction by the exercising legs was positively correlated to arterial lactate concentration (r = 0.81, P less than 0.001) at the lower two power outputs. The arms showed net lactate extraction during exercise, which was correlated to the arterial concentration (r = 0.86). The results demonstrate that exercising skeletal muscle extracts a significant amount of lactate during net lactate release and that the working skeletal muscle appears to be a major site of blood lactate removal during exercise.
Authors:
W C Stanley; E W Gertz; J A Wisneski; R A Neese; D L Morris; G A Brooks
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  60     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-06-09     Completed Date:  1986-06-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1116-20     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Transport, Active
Carbon Dioxide / metabolism
Humans
Lactates / blood,  metabolism*,  secretion
Lactic Acid
Leg
Male
Muscles / metabolism*
Oxidation-Reduction
Physical Exertion*
Grant Support
ID/Acronym/Agency:
AM-19577/AM/NIADDK NIH HHS; HL-25625/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Lactates; 124-38-9/Carbon Dioxide; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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