Document Detail


Lactate distribution and metabolism during and after anoxia in the turtle, Chrysemys picta bellii.
MedLine Citation:
PMID:  8770142     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the fate of lactate during and after prolonged anoxia, 14C-labeled lactate was injected into turtles after 2 h of a 6-h submergence at 20 degrees C. 14C activities of plasma and chamber water were tested at intervals during anoxia and also in expired air during 39 h of recovery. Partitioning of label in major body compartments [extracellular fluid (ECF), intracellular fluid (ICF), and shell] and 14C activity and glycogen in selected tissues (heart, liver, and muscle) were measured after anoxia (n = 7) and after recovery (n = 6). Shell 14C and [lactate] were extensively measured on six anoxic turtles. During anoxia all 14C remained in the animal indicating no urine production. At 6 h of anoxia 47% of recovered 14C, presumably still as lactate, was in the ECF, 27% in the ICF, and 30% in the shell. During recovery, plasma [lactate] fell from 35 to 5 meq, but surrounding water and expired air accounted for only 9 and 8%, respectively, of recovered label. The ICF portion grew to 41%, associated with a recovery in tissue glycogen. The shell still had 22% of total label. We conclude that, during recovery from anoxia, lactate is predominantly resynthesized to glycogen, and only a small fraction is directly oxidized. During anoxia, however, lactate is widely distributed in the body, and a surprisingly large and functionally significant fraction resides in the shell.
Authors:
D C Jackson; V I Toney; S Okamoto
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  271     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-30     Completed Date:  1996-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R409-16     Citation Subset:  IM    
Affiliation:
Department of Physiology, Brown University, Providence, Rhode Island 02912, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / metabolism*
Carbon Dioxide
Carbon Radioisotopes
Extracellular Space / metabolism
Glycogen / metabolism
Lactic Acid / blood,  metabolism*
Liver / metabolism
Muscles / metabolism
Myocardium / metabolism
Respiration
Tissue Distribution
Turtles / metabolism*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 124-38-9/Carbon Dioxide; 50-21-5/Lactic Acid; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Glycogen depletion of the intrafusal fibers in a mouse muscle spindle during prolonged swimming.
Next Document:  Cardiac performance in relation to oxygen supply varies with dietary lipid composition in sturgeon.