| Lactate distribution and metabolism during and after anoxia in the turtle, Chrysemys picta bellii. | |
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MedLine Citation:
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PMID: 8770142 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the fate of lactate during and after prolonged anoxia, 14C-labeled lactate was injected into turtles after 2 h of a 6-h submergence at 20 degrees C. 14C activities of plasma and chamber water were tested at intervals during anoxia and also in expired air during 39 h of recovery. Partitioning of label in major body compartments [extracellular fluid (ECF), intracellular fluid (ICF), and shell] and 14C activity and glycogen in selected tissues (heart, liver, and muscle) were measured after anoxia (n = 7) and after recovery (n = 6). Shell 14C and [lactate] were extensively measured on six anoxic turtles. During anoxia all 14C remained in the animal indicating no urine production. At 6 h of anoxia 47% of recovered 14C, presumably still as lactate, was in the ECF, 27% in the ICF, and 30% in the shell. During recovery, plasma [lactate] fell from 35 to 5 meq, but surrounding water and expired air accounted for only 9 and 8%, respectively, of recovered label. The ICF portion grew to 41%, associated with a recovery in tissue glycogen. The shell still had 22% of total label. We conclude that, during recovery from anoxia, lactate is predominantly resynthesized to glycogen, and only a small fraction is directly oxidized. During anoxia, however, lactate is widely distributed in the body, and a surprisingly large and functionally significant fraction resides in the shell. |
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Authors:
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D C Jackson; V I Toney; S Okamoto |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-10-30 Completed Date: 1996-10-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R409-16 Citation Subset: IM |
Affiliation:
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Department of Physiology, Brown University, Providence, Rhode Island 02912, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / metabolism* Carbon Dioxide Carbon Radioisotopes Extracellular Space / metabolism Glycogen / metabolism Lactic Acid / blood, metabolism* Liver / metabolism Muscles / metabolism Myocardium / metabolism Respiration Tissue Distribution Turtles / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 124-38-9/Carbon Dioxide; 50-21-5/Lactic Acid; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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