| Lactate damages primary hippocampal neurons in vitro. | |
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MedLine Citation:
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PMID: 19947934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, rat primary cultures were used to study the effect of lactate on the survival of hippocampal neurons in the presence or absence of glucose. Our results showed no extensive cell damage under glucose-free conditions compared with glucose-rich conditions. Addition of 10 and 50 mM lactate to glucose-free and glucose-rich media increased the cell damage significantly, as observed by morphology and lactate dehydrogenase activity. The results of the present study suggest that primary neurons in vitro are not sensitive to glucose deficiency and the presence of lactate damages the neurons in a concentration-dependent manner. |
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Authors:
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Arian Sepehr; Simin Mohseni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-16 |
Journal Detail:
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Title: Cell biology international Volume: 34 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-05-04 Revised Date: 2010-11-12 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 61-5 Citation Subset: IM |
Affiliation:
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Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping 58185, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Embryo, Mammalian / cytology Glucose / pharmacology Hippocampus / cytology* Lactate Dehydrogenases / metabolism Lactates / toxicity* Neurons / cytology, drug effects, metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 50-99-7/Glucose; EC 1.1.-/Lactate Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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