| Lack of tropomyosin correlates with the absence of stress fibers in transformed rat kidney cells. | |
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MedLine Citation:
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PMID: 6282338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have utilized epithelial rat kidney cells and their Kirsten viral transformant (442) to examine the role of actin-binding proteins in cellular morphogenesis. Normal rat kidney cells are well spread while the transformed cells are more spherical, poorly adherent, and lack actin stress fibers (Rubin, R.W., Warren, R.H., Lukeman, D.S. and Clements, E. (1978) J. Cell Biol. 78, 28-35). By immunofluorescence, antitropomyosin prominently stains normal rat kidney cell stress fibers while only a weak, nonspecific fluorescence is observed in 442 cells. Using two-dimensional gel electrophoresis, tropomyosin can be detected in normal rat kidney cells homogenates. The tropomyosin subunits are enriched in Triton-extracted filamentous normal rat kidney cell models, and in extracts of normal rat kidney cell homogenate produced by using a rapid myosin affinity technique to isolate actin and actin-associated proteins. The identity of the tropomyosin subunits has been confirmed by electrophoretic mobility, lack of proline, and the peptide map generated by limited proteolysis. None of these techniques have detected tropomyosin in the corresponding 442 preparations. Our results suggest that the transformation of normal rat kidney cells has led to an overall reduction in tropomyosin content. This may be related to the inability of 442 cells to organize filamentous actin stress fibers. |
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Authors:
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C L Leonardi; R H Warren; R W Rubin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 720 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1982 Apr |
Date Detail:
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Created Date: 1982-08-07 Completed Date: 1982-08-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 154-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cell Transformation, Viral* Cells, Cultured Electrophoresis, Polyacrylamide Gel Kidney / physiology* Kirsten murine sarcoma virus / genetics* Molecular Weight Peptide Fragments / analysis Rats Sarcoma Viruses, Murine / genetics* Tropomyosin / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Peptide Fragments; 0/Tropomyosin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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