Document Detail

Lack of transport of erythropoietin across the human placenta as studied by an in vitro perfusion system.
MedLine Citation:
PMID:  8058465     Owner:  NLM     Status:  MEDLINE    
The transfer of human recombinant erythropoietin (rhEPO) from the maternal to the fetal side was investigated using the technique of in vitro perfusion of an isolated cotyledon of human placenta, with recirculation of the perfusate (130 ml) in separate closed maternal and fetal circuits. rhEPO (221-512 U), together with [14C]BSA (bovine serum albumin, 44.8 kBq or 2,688,000 dpm), was added to the maternal circuit only. Despite a considerably lower molecular weight of EPO (mol. wt. = 30,400 Da) compared to BSA (mol. wt. = 69,000 Da), no difference was found in their transfer across the placenta from the maternal to the fetal side, which was very low for both macromolecules. The total transfer of rhEPO derived from the concentration measured in the samples taken from the fetal circuit at the end of 4-5 h of perfusion, was in the range of 0.04% of the amount initially added to the maternal compartment. A similar amount of transfer was determined for [14C]BSA (0.04-0.07%, n = 12). In conclusion, by direct determination in a dually in vitro perfused human placental cotyledon, no significant transfer of rhEPO from the maternal to the fetal side could be shown.
A Malek; R Sager; K U Eckardt; C Bauer; H Schneider
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  427     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-09-12     Completed Date:  1994-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  157-61     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Berne, Switzerland.
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MeSH Terms
Biological Transport
Erythropoietin / blood,  metabolism*
Fetal Blood
Maternal-Fetal Exchange*
Osmolar Concentration
Placenta / metabolism*
Serum Albumin, Bovine / pharmacokinetics
Reg. No./Substance:
0/Serum Albumin, Bovine; 11096-26-7/Erythropoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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