| Lack of transport of erythropoietin across the human placenta as studied by an in vitro perfusion system. | |
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MedLine Citation:
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PMID: 8058465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transfer of human recombinant erythropoietin (rhEPO) from the maternal to the fetal side was investigated using the technique of in vitro perfusion of an isolated cotyledon of human placenta, with recirculation of the perfusate (130 ml) in separate closed maternal and fetal circuits. rhEPO (221-512 U), together with [14C]BSA (bovine serum albumin, 44.8 kBq or 2,688,000 dpm), was added to the maternal circuit only. Despite a considerably lower molecular weight of EPO (mol. wt. = 30,400 Da) compared to BSA (mol. wt. = 69,000 Da), no difference was found in their transfer across the placenta from the maternal to the fetal side, which was very low for both macromolecules. The total transfer of rhEPO derived from the concentration measured in the samples taken from the fetal circuit at the end of 4-5 h of perfusion, was in the range of 0.04% of the amount initially added to the maternal compartment. A similar amount of transfer was determined for [14C]BSA (0.04-0.07%, n = 12). In conclusion, by direct determination in a dually in vitro perfused human placental cotyledon, no significant transfer of rhEPO from the maternal to the fetal side could be shown. |
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Authors:
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A Malek; R Sager; K U Eckardt; C Bauer; H Schneider |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 427 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-09-12 Completed Date: 1994-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 157-61 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Berne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Erythropoietin / blood, metabolism* Female Fetal Blood Humans Maternal-Fetal Exchange* Osmolar Concentration Perfusion Permeability Placenta / metabolism* Pregnancy Serum Albumin, Bovine / pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Serum Albumin, Bovine; 11096-26-7/Erythropoietin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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