| Lack of a protective effect of insulin on three reperfusion-liver injury models in rats and mice. | |
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MedLine Citation:
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PMID: 16980048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our objective was to investigate the potential protective effects of insulin on the liver injury induced in three ischemia and reperfusion (I/R) models. METHODS: Three I/R models were used: (1) I/R of the liver was produced in isolated, perfused rat livers; (2) in in situ I/R of the liver in rats, ischemia was induced by clamping off the hepatic artery and portal vein for 40 minutes, the flow then restored, and the liver reperfused for 90 minutes; (3) in in situ I/R of the liver in mice, ischemia was induced by clamping off the hepatic artery for 15 minutes, the flow then restored, and the liver reperfused for 45 minutes. In all three cases, blood samples collected before ischemia and after reperfusion were analyzed for sGOT. Plasma nitrate/nitrite, hydroxyl radicals, and tumor necrosis factor were also measured. In each model, a dose of insulin sufficient to induce euglycemia was administered to assess its protective effect on liver injury and inflammation. RESULTS: These I/R protocols resulted in a significant increase in sGOT and in three inflammatory parameters; nitric oxide, hydroxyl radicals, and tumor necrosis factor. Pretreatment with insulin did not attenuate the liver injury in any of the three I/R models. CONCLUSIONS: Although insulin has been reported to provide anti-inflammatory benefits by reducing oxidative and nitrosative stress and cytokine release, none of these protective effects was seen in the three I/R-induced liver injury models we tested. |
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Authors:
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C F Chen; F J Leu; H I Chen; D Wang; S J Chou |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 38 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-18 Completed Date: 2006-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 2221-5 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology, Department of Internal Medicine, Cheng Hsin General Hospital, and School of Health, Ming Chuan University, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartate Aminotransferases / blood Blood Flow Velocity / drug effects Disease Models, Animal Hepatic Artery Insulin / pharmacology* Liver Circulation / drug effects, physiology* Liver Function Tests Male Mice Rats Rats, Sprague-Dawley Reperfusion Injury / physiopathology* |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; EC 2.6.1.1/Aspartate Aminotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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