Document Detail


Lack of mRNA and dystrophin expression in DMD patients three months after myoblast transfer.
MedLine Citation:
PMID:  7580241     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report our experience on myoblast transplantation in three Duchenne muscular dystrophy patients. Pure myoblasts (55 x 10(6) per patient) from HLA-matched donors, were injected into a tibialis anterior and the controlateral muscle was sham injected. Three months after transplantation, biopsies from the injected muscles were negative for dystrophin expression by immunocytochemistry. Reverse transcriptase-PCR (RT-PCR) failed to amplify any fragments of the deleted regions. This result confirms that myoblast transplantation is feasible, although the efficacy of this therapeutic approach is poor.
Authors:
L Morandi; P Bernasconi; M Gebbia; M Mora; F Crosti; R Mantegazza; F Cornelio
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuromuscular disorders : NMD     Volume:  5     ISSN:  0960-8966     ISO Abbreviation:  Neuromuscul. Disord.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-11-27     Completed Date:  1995-11-27     Revised Date:  2012-02-27    
Medline Journal Info:
Nlm Unique ID:  9111470     Medline TA:  Neuromuscul Disord     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  291-5     Citation Subset:  IM    
Affiliation:
Department of Neuromuscular Diseases, Istituto Nazionale Neurologico C. Besta, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Biopsy
Blotting, Western
Child
DNA / analysis
Dystrophin / analysis*,  genetics
Humans
Injections, Intramuscular
Male
Molecular Sequence Data
Muscle Fibers, Skeletal / transplantation*
Muscular Dystrophies / therapy*
Polymerase Chain Reaction
RNA, Messenger / analysis
Time Factors
Grant Support
ID/Acronym/Agency:
20//Telethon; 21//Telethon
Chemical
Reg. No./Substance:
0/Dystrophin; 0/RNA, Messenger; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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