Document Detail

Lack of inhibition by dideoxy-forskolin and verapamil of DIDS-sensitive volume-activated Cl- secretion in human squamous lung carcinoma epithelial cells.
MedLine Citation:
PMID:  7803488     Owner:  NLM     Status:  MEDLINE    
The effect of osmotic stress on Cl- permeability in human squamous lung carcinoma epithelial (S1) cells was investigated using a macroscopic 125I efflux assay. Hypotonic challenge of monolayers led to a significant (P < 0.01) dose-related increase in efflux from pre-loaded cells, returning to pre-activation rates within 10 min. A similar magnitude of response could be produced by challenge with an isotonic low chloride-containing solution. Neither 100 mM dideoxy-forskolin nor 100 mM verapamil inhibited the increase in Cl- secretion after hypotonic challenge, whereas 100 mM DIDS inhibited volume-activated Cl- secretion by 55%. Both Northern and Western blot analysis confirmed the absence of MDR1 mRNA and P-glycoprotein in the S1 cells. We conclude that these cells have a volume-regulated Cl- secretory pathway that is independent of the ABC transporter, P-glycoprotein.
F M Munkonge; L R Osborne; D M Geddes; E W Alton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1224     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-02-02     Completed Date:  1995-02-02     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  342-8     Citation Subset:  IM    
Ion Transport Unit, National Heart and Lung Institute, London, UK.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology*
Blotting, Northern
Blotting, Western
Carcinoma, Squamous Cell / secretion*
Cell Size
Chlorides / metabolism*
Forskolin / analogs & derivatives*,  pharmacology
Lung Neoplasms / secretion*
Osmolar Concentration
P-Glycoprotein / genetics
Tumor Cells, Cultured
Verapamil / pharmacology*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Chlorides; 0/P-Glycoprotein; 52-53-9/Verapamil; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 64657-18-7/1,9-dideoxyforskolin; 66428-89-5/Forskolin

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